Department of Physiology, Genetics, and Microbiology, University of Alicante, Alicante, Spain.
Flow Cytometry Unit: Pompeu Fabra University (UPF) and Centre for Genomic Regulation (CRG), The Barcelona Institute for Sciences and Technology (BIST), Barcelona, Spain.
ISME J. 2019 Jan;13(1):232-236. doi: 10.1038/s41396-018-0278-7. Epub 2018 Sep 18.
The identification of relevant virus-host pairs that globally account for a large pool of carbon and nutrients in the ocean is paramount to build accurate ecological models. A previous work using single-virus genomics led to the discovery of the uncultured single-virus vSAG 37-F6, originally sorted from the Mediterranean Sea (Blanes Bay Microbial Observatory), that represents one of the most abundant dsDNA viral population in the marine surface virosphere. Here, from same sampling site, we report that a Pelagibacter single-cell contained a viral member of vSAG 37-F6 population, by means of PCR screening of sorted, genome-amplified single cells with vSAG 37-F6-specific primers and whole-genome sequencing. Furthermore, viruses from this population were also found in three other Pelagibacter single cells from the South Pacific and Atlantic oceans. These new uncultured pelagiphages were genetically different from the previously characterized pelagiphage isolates. Data showed that the uncultured vSAG 37-F6 population represents the Pelagibacter phages that inhabit the sunlit ocean better, and contains a vast unrecognized microdiversity.
鉴定在全球海洋中具有大量碳和营养物质的相关病毒-宿主对，对于构建准确的生态模型至关重要。之前的一项使用单病毒基因组学的研究发现了未培养的单病毒 vSAG 37-F6，该病毒最初是从地中海（布兰斯湾微生物观测站）中分离出来的，它是海洋表面病毒圈中最丰富的双链 DNA 病毒群之一。在这里，我们从同一采样点报告说，通过使用 vSAG 37-F6 特异性引物对经分选和基因组扩增的单个细胞进行 PCR 筛选，并对全基因组进行测序，从 Pelagibacter 单细胞中发现了 vSAG 37-F6 种群的病毒成员。此外，在南太平洋和大西洋的另外三个 Pelagibacter 单细胞中也发现了来自该种群的病毒。这些新的未培养的浮游噬菌体在遗传上与之前表征的浮游噬菌体分离物不同。数据表明，未培养的 vSAG 37-F6 种群更能代表栖息在阳光充足的海洋中的 Pelagibacter 噬菌体，并且包含大量未被识别的微多样性。
