Department of Physiology, Genetics, and Microbiology, University of Alicante, Carretera San Vicente del Raspeig, San Vicente del Raspeig, Alicante 03690, Spain.
Centre for Genomic Regulation (CRG), The Barcelona Institute for Science and Technology (BIST), Carrer del Doctor Aiguader, 88, PRBB Building, Barcelona 08003, Spain.
Nat Commun. 2017 Jun 23;8:15892. doi: 10.1038/ncomms15892.
Microbes drive ecosystems under constraints imposed by viruses. However, a lack of virus genome information hinders our ability to answer fundamental, biological questions concerning microbial communities. Here we apply single-virus genomics (SVGs) to assess whether portions of marine viral communities are missed by current techniques. The majority of the here-identified 44 viral single-amplified genomes (vSAGs) are more abundant in global ocean virome data sets than published metagenome-assembled viral genomes or isolates. This indicates that vSAGs likely best represent the dsDNA viral populations dominating the oceans. Species-specific recruitment patterns and virome simulation data suggest that vSAGs are highly microdiverse and that microdiversity hinders the metagenomic assembly, which could explain why their genomes have not been identified before. Altogether, SVGs enable the discovery of some of the likely most abundant and ecologically relevant marine viral species, such as vSAG 37-F6, which were overlooked by other methodologies.
微生物在病毒施加的限制下驱动着生态系统的运转。然而，由于缺乏病毒基因组信息，我们无法回答有关微生物群落的基本生物学问题。在这里，我们应用单病毒基因组学（SVGs）来评估当前技术是否遗漏了海洋病毒群落的部分内容。在这里鉴定的 44 个病毒单扩增基因组（vSAGs）中，大多数在全球海洋病毒组数据集中的丰度都高于已发表的宏基因组组装病毒基因组或分离株。这表明 vSAGs 可能最能代表主导海洋的 dsDNA 病毒种群。物种特异性招募模式和病毒组模拟数据表明，vSAGs 高度多样化，微多样性阻碍了宏基因组组装，这可能解释了为什么以前没有发现它们的基因组。总的来说，SVGs 能够发现一些可能最丰富和具有生态相关性的海洋病毒物种，例如 vSAG 37-F6，这是其他方法所忽略的。
