Bober E, Singh S, Agarwal D P, Goedde H W
Biosci Rep. 1986 Jul;6(7):633-8. doi: 10.1007/BF01114757.
Human fetal muscle cDNA library was screened with a beta-myosin heavy chain gene fragment containing Alu sequences. Two cDNA clones AI and BII with 1.8 and 3 kb inserts respectively were chosen for further characterization by means of RNA and DNA hybridization procedures and sequencing. The clones appeared to contain repetitive sequences as well as single copy regions. They are actively transcribed in different stages of myogenic development but not in the liver. DNA sequence analysis of short stretches from both clones revealed no sequence homology to any other published DNA sequences.
用人β-肌球蛋白重链基因含Alu序列的片段筛选人胎儿肌肉cDNA文库。挑选出两个分别带有1.8kb和3kb插入片段的cDNA克隆AI和BII，通过RNA和DNA杂交程序及测序进行进一步鉴定。这些克隆似乎既含有重复序列也含有单拷贝区域。它们在肌源性发育的不同阶段都有活跃转录，但在肝脏中不转录。对两个克隆的短片段进行DNA序列分析，结果显示与其他已发表的DNA序列没有序列同源性。
