Robert B, Weydert A, Caravatti M, Minty A, Cohen A, Daubas P, Gros F, Buckingham M
Proc Natl Acad Sci U S A. 1982 Apr;79(8):2437-41. doi: 10.1073/pnas.79.8.2437.
A recombinant plasmid with a cDNA sequence transcribed from mouse skeletal muscle RNA is shown to hybridize with mRNAs for myosin light chains LC1F and LC3F. The inserted fragment corresponds exclusively to the 3'-noncoding region of the mRNA. It hybridizes almost exclusively with the two light chain messengers from fast skeletal muscle RNA of adult mouse. Slight hybridization is seen with RNA from heart muscle and embryonic skeletal muscle. The implications of the conservation of the 3'-noncoding regions between the two mRNAs are discussed.
一个含有从小鼠骨骼肌RNA转录而来的cDNA序列的重组质粒,被证明可与肌球蛋白轻链LC1F和LC3F的mRNA杂交。插入片段仅对应于mRNA的3'-非编码区。它几乎只与成年小鼠快骨骼肌RNA中的两种轻链信使杂交。在心肌和胚胎骨骼肌的RNA中可见轻微杂交。讨论了两种mRNA之间3'-非编码区保守性的意义。