Laboratoire Transformations Intégrées de la Matière Renouvelable (UTC/ESCOM, EA 4297 TIMR) Centre de recherche Royallieu, Université de Technologie de Compiègne, Sorbonne Universités, CS 60319, 60203, Compiègne Cedex, France.
LUNAM Université, CNRS, GEPEA, Université de Nantes, UMR6144, CRTT, Boulevard de l'Université, BP 406, 44602, Saint-Nazaire Cedex, France.
Bioprocess Biosyst Eng. 2019 Jan;42(1):29-36. doi: 10.1007/s00449-018-2010-4. Epub 2018 Sep 18.
Treatments with high-voltage electrical discharges (HVED) and high-pressure homogenization (HPH) were studied and compared for the release of ionic components, carbohydrates, proteins, and pigments from microalgae Parachlorella kessleri (P. kessleri). Suspensions (1% w/w) of microalgae were treated by HVED (40 kV/cm, 1-8 ms) or by HPH (400-1200 bar, 1-10 passes). Particle-size distribution (PSD) and microscopic analyses were used to detect the disruption and damage of cells. HVED were very effective for the extraction of ionic cell components and carbohydrates (421 mg/L after 8 ms of the treatment). However, HVED were ineffective for pigments and protein extraction. The concentration of proteins extracted by HVED was just 750 mg/L and did not exceed 15% of the total quantity of proteins. HPH permitted an effective release overall of intracellular compounds from P. kessleri microalgae including a large quantity of proteins, whose release (at 1200 bar) was 4.9 times higher than that obtained by HVED. Consequently, HVED can be used at the first step of the overall extraction process for the selective recovery of low-molecular-weight components. HPH can be then used at the second step for the recovery of remaining cell compounds.
研究了高压放电（HVED）和高压匀浆（HPH）处理方法，并对其从微藻 Parachlorella kessleri（P. kessleri）中释放离子成分、碳水化合物、蛋白质和色素的效果进行了比较。将微藻悬浮液（1%w/w）用 HVED（40 kV/cm，1-8 ms）或 HPH（400-1200 bar，1-10 次通过）处理。采用颗粒大小分布（PSD）和显微镜分析来检测细胞的破坏和损伤。HVED 非常有效地提取离子细胞成分和碳水化合物（处理 8 ms 后达到 421 mg/L）。然而，HVED 对色素和蛋白质的提取无效。HVED 提取的蛋白质浓度仅为 750 mg/L，且不超过蛋白质总量的 15%。HPH 允许从 P. kessleri 微藻中有效释放总体细胞内化合物，包括大量蛋白质，其释放量（在 1200 bar 下）比 HVED 高 4.9 倍。因此，HVED 可用于整个提取过程的第一步，以选择性回收低分子量成分。然后，HPH 可用于第二步，以回收剩余的细胞化合物。
