Sorbonne University, Université de Technologie de Compiègne, ESCOM, EA 4297 TIMR, Centre de Recherche Royallieu, CS 60319, 60203 Compiègne Cedex, France.
LUNAM Université, CNRS, GEPEA, Université de Nantes, UMR6144, CRTT, Boulevard de l'Université, BP 406, 44602 Saint-Nazaire Cedex, France.
Bioresour Technol. 2020 Apr;302:122893. doi: 10.1016/j.biortech.2020.122893. Epub 2020 Jan 25.
Two-step procedure with the initial aqueous extraction from raw microalgae Nannochloropsis oculata and secondary organic solvent extraction from vacuum dried (VD) microalgae were applied for selective recovery of bio-molecules. The effects of preliminary aqueous washing and high voltage electrical discharges (HVED, 40 kV/cm, 4 ms pulses) were tested. The positive effects of HVED treatment and washing on selectivity of aqueous extraction of ionics and other water-soluble compounds (carbohydrates, proteins and pigments) were observed. Moreover, the HVED treatment allowed improving the kinetic of vacuum drying, and significant effects of HVED treatment on organic solvent extraction of chlorophylls, carotenoids and lipids were determined. The proposed two-step procedure combining the preliminary washing, HVED treatment and aqueous/organic solvents extraction steps are useful for selective extraction of different bio-molecules from microalgae biomass.
两步法，先用初始的水性萃取从原始微藻中提取，再用有机溶剂从真空干燥（VD）微藻中提取，用于选择性回收生物分子。本文测试了初步的水性洗涤和高压电放电（HVED，40kV/cm，4ms 脉冲）的效果。HVED 处理和洗涤对离子和其他水溶性化合物（碳水化合物、蛋白质和色素）的水性萃取选择性有积极影响。此外，HVED 处理还可以改善真空干燥的动力学，并且确定 HVED 处理对叶绿素、类胡萝卜素和脂质的有机溶剂萃取有显著影响。该两步法结合了初步洗涤、HVED 处理和水/有机溶剂萃取步骤，可用于从微藻生物质中选择性提取不同的生物分子。
