Johnson D, Lanahan A, Buck C R, Sehgal A, Morgan C, Mercer E, Bothwell M, Chao M
Cell. 1986 Nov 21;47(4):545-54. doi: 10.1016/0092-8674(86)90619-7.
The nucleotide sequence for the human nerve growth factor (NGF) receptor has been determined. The 3.8 kb receptor mRNA encodes a 427 amino acid protein containing a 28 amino acid signal peptide, an extracellular domain containing four 40 amino acid repeats with six cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155 amino acid cytoplasmic domain. The sequence of the extracellular domain of the NGF receptor predicts a highly ordered structure containing a negatively charged region that may serve as the ligand-binding site. This domain is conserved through evolution. Transfection of a full-length cDNA in mouse fibroblasts results in stable expression of NGF receptors that are recognized by monoclonal antibodies to the human NGF receptor and that bind [125I]NGF.
已确定人类神经生长因子（NGF）受体的核苷酸序列。3.8 kb的受体mRNA编码一种含427个氨基酸的蛋白质，该蛋白质含有一个28个氨基酸的信号肽、一个细胞外结构域，此结构域包含四个由40个氨基酸组成的重复序列，在保守位置有六个半胱氨酸残基，随后是一个富含丝氨酸/苏氨酸的区域、一个单一的跨膜结构域以及一个含155个氨基酸的胞质结构域。NGF受体细胞外结构域的序列预测其具有高度有序的结构，其中包含一个可能作为配体结合位点的带负电荷区域。该结构域在进化过程中是保守的。将全长cDNA转染到小鼠成纤维细胞中可导致NGF受体的稳定表达，这些受体可被针对人类NGF受体的单克隆抗体识别并能结合[125I]NGF。
