Large T H, Weskamp G, Helder J C, Radeke M J, Misko T P, Shooter E M, Reichardt L F
Department of Physiology, University of California, San Francisco 94143-0724.
Neuron. 1989 Feb;2(2):1123-34. doi: 10.1016/0896-6273(89)90179-7.
Chicken nerve growth factor (NGF) receptor cDNAs have been isolated and sequenced in an effort to identify functionally important receptor domains and as an initial step in determining the functions of the NGF receptor in early embryogenesis. Comparisons of the primary amino acid sequences of the avian and mammalian NGF receptors have identified several discrete domains that differ in their degree of conservation. The highly conserved regions include an extracellular domain, likely to be involved in ligand binding, in which the positions of 24 cysteine residues and virtually all negatively charged residues are conserved; a transmembrane region, including flanking stretches of extracellular and cytoplasmic amino acids, which has properties suggesting it interacts with other proteins; and a cytoplasmic PEST sequence, which may regulate receptor turnover. Transient expression of NGF receptor mRNA has been seen in many regions of the developing CNS. Experiments suggest that both NGF and its receptor help regulate development of the retina.
为了鉴定功能上重要的受体结构域，并作为确定神经生长因子（NGF）受体在早期胚胎发生中的功能的第一步，鸡神经生长因子受体的cDNA已被分离和测序。对鸟类和哺乳动物NGF受体的一级氨基酸序列进行比较，确定了几个在保守程度上不同的离散结构域。高度保守的区域包括一个可能参与配体结合的细胞外结构域，其中24个半胱氨酸残基和几乎所有带负电荷残基的位置都是保守的；一个跨膜区域，包括细胞外和细胞质氨基酸的侧翼延伸，其特性表明它与其他蛋白质相互作用；以及一个细胞质PEST序列，它可能调节受体的更新。在发育中的中枢神经系统的许多区域都观察到了NGF受体mRNA的瞬时表达。实验表明，NGF及其受体都有助于调节视网膜的发育。
