Bailey-Serres J, Hanson D K, Fox T D, Leaver C J
Cell. 1986 Nov 21;47(4):567-76. doi: 10.1016/0092-8674(86)90621-5.
The mitochondrial gene (COXI) encoding cytochrome c oxidase subunit I (COI) was isolated from two cytoplasmic genotypes of sorghum that synthesize different COI polypeptides. The Milo COI (Mr approximately 38,000) is encoded by a 530 codon structural gene sharing 98% homology with the corresponding maize gene. A variant COI observed in 9E cytoplasm (Mr approximately 42,000) is encoded by a 631 codon structural gene that diverges completely from the Milo COXI gene both 100 bp 5' to the presumed initiator methionine and within the 3' coding sequence. The 3' divergence results in a 101 C-terminal extension of the 9E COI that is not homologous to any known mitochondrial polypeptide. The novel 9E COXI, apparently arising from at least two rearrangements, affects transcription and gene product.
从两种合成不同细胞色素c氧化酶亚基I（COI）多肽的高粱细胞质基因型中分离出编码细胞色素c氧化酶亚基I（COI）的线粒体基因（COXI）。米洛COI（约38,000道尔顿）由一个530密码子的结构基因编码，与相应的玉米基因具有98%的同源性。在9E细胞质中观察到的一种变异COI（约42,000道尔顿）由一个631密码子的结构基因编码，该基因在假定起始甲硫氨酸的5'端100 bp处以及3'编码序列内与米洛COXI基因完全不同。3'端的差异导致9E COI的C末端有101个氨基酸的延伸，这与任何已知的线粒体多肽都不同源。这种新的9E COXI显然是由至少两次重排产生的，它影响转录和基因产物。
