Grabau E A
Howard Hughes Medical Institute, University of Utah, Salt Lake City 84132.
Curr Genet. 1987;11(4):287-93. doi: 10.1007/BF00355402.
The nucleotide sequence of the cytochrome oxidase subunit II (COII) gene from soybean mitochondria reveals a high degree of sequence conservation to other plant COII sequences. The greatest homology is to pea (97.9%) and the homology extends for some distance both 5' and 3' from the gene. Upstream from the breakpoint in homology with the pea sequence there is a tRNA gene identified as the initiator methionine by homology to known plant tRNA genes. The COII coding sequence appears to be present at a single location in the soybean mitochondrial genome, however some portion of the 5' flanking region is repeated at other sites in the genome. The transcription from the cloned region is complex and is complicated by the presence of the repeated sequence.
大豆线粒体细胞色素氧化酶亚基II(COII)基因的核苷酸序列显示出与其他植物COII序列高度的序列保守性。与豌豆的同源性最高(97.9%),并且同源性在该基因的5'和3'端都延伸了一段距离。在与豌豆序列同源性中断点的上游,有一个tRNA基因,通过与已知植物tRNA基因的同源性鉴定为起始甲硫氨酸tRNA基因。COII编码序列似乎存在于大豆线粒体基因组的单个位置,然而5'侧翼区域的某些部分在基因组的其他位点重复。克隆区域的转录很复杂,并且由于重复序列的存在而变得更加复杂。