Programa de Pós-Graduação em Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Metab Brain Dis. 2018 Dec;33(6):2059-2063. doi: 10.1007/s11011-018-0311-6. Epub 2018 Sep 18.
Leptin is an adipose tissue-derived hormone that acts on the hypothalamus in order to maintain energy homeostasis. However, leptin can also induce an inflammatory response. Increasing evidence has highlighted a critical role of astrocytes in the effects of leptin on the hypothalamus. In addition, astrocytes participate in neuroinflammation by producing and releasing a wide range of inflammatory mediators. In this study, we aimed to investigate the age-dependent effect of leptin on pro- and anti-inflammatory cytokines released by the hypothalamic astrocyte cultures obtained from newborn, adult, and aged Wistar rats. In hypothalamic astrocytes from newborn rats, leptin did not change the release of pro-inflammatory cytokines, tumor necrosis factor α (TNF-α) and interleukin 1β (IL-1β). On the other contrary, leptin increased the release of both TNF-α and IL-1β in astrocyte cultures from adult and aged animals. Regarding the anti-inflammatory cytokine interleukin 10 (IL-10), we did not observe any change in response to leptin. In conclusion, our data suggests a pro-inflammatory action of leptin on the hypothalamus during aging. This in turn may be related to the triggering of metabolic disorders, as both of these conditions are associated with neuroinflammation.
瘦素是一种脂肪组织来源的激素，作用于下丘脑以维持能量平衡。然而，瘦素也可以引发炎症反应。越来越多的证据强调了星形胶质细胞在瘦素对下丘脑作用中的关键作用。此外，星形胶质细胞通过产生和释放广泛的炎症介质参与神经炎症。在这项研究中，我们旨在研究瘦素对从新生、成年和老年 Wistar 大鼠下丘脑星形胶质细胞培养物中释放的促炎和抗炎细胞因子的年龄依赖性影响。在新生大鼠的下丘脑星形胶质细胞中，瘦素并未改变促炎细胞因子肿瘤坏死因子-α（TNF-α）和白细胞介素 1β（IL-1β）的释放。相反，瘦素增加了成年和老年动物星形胶质细胞培养物中两种细胞因子 TNF-α和 IL-1β的释放。关于抗炎细胞因子白细胞介素 10（IL-10），我们没有观察到瘦素的任何反应变化。总之，我们的数据表明，瘦素在衰老过程中对下丘脑具有促炎作用。这反过来可能与代谢紊乱的触发有关，因为这两种情况都与神经炎症有关。
