Research group of Functional Food Materials, Research Division of Food Functionality, Korea Food Research Institute (KFRI), 245, Nongsaengmyeong-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do, 55365, Republic of Korea.
Metab Brain Dis. 2018 Dec;33(6):1877-1886. doi: 10.1007/s11011-018-0294-3. Epub 2018 Sep 18.
Dried Citrus unshiu peel, also known as Chinpi, have been commonly used as a traditional medicine to improve for allergy, inflammation and hepatopathy. Many previously studies have reported that citrus flavonoids show neuroprotective activities. However, the antidepressant-related effects of C. unshiu peels have not been well characterized. Here, the antidepressant-like effects of standardized C. unshiu peel extract (SCP) were evaluated in in vivo and in vitro depression models induced by dexamethasone (DEX), a synthetic glucocorticoid. Male ICR mice (9-week-old) were injected the DEX (40 mg/kg) and were orally given SCP daily (30, 100, and 300 mg/kg) for 14 consecutive days. The depressive-like behaviors were determined by use of open filed test (OFT), sucrose preference test (SPT), tail suspension test (TST), and forced swim test (FST). We show that treatment with SCP significantly alleviated DEX-induced depressive-like behaviors and reduced neurotoxicity in a concentration dependent manner in SH-SY5Y cells. Additionally, repeated DEX injection markedly decreased brain derived neurotrophic factor (BDNF) level, tropomyosin receptor kinase B (TrkB), and cyclic AMP-response element-binding protein (CREB), while SCP treatment improved these levels in the cerebral cortex and hippocampus regions. Our findings suggest that SCP exhibits significant antidepressant-like effects in the DEX-induced depressive animal model, and this activity may be mediated by preventing corticosterone-induced neurotoxicity.
陈皮，又名 Chinpi，作为一种传统药物，常用于改善过敏、炎症和肝病。许多先前的研究报告称，柑橘类黄酮具有神经保护活性。然而，陈皮对抑郁症的相关作用尚未得到很好的描述。在这里，我们评估了标准化陈皮提取物（SCP）在由地塞米松（DEX）诱导的体内和体外抑郁模型中的抗抑郁样作用，DEX 是一种合成糖皮质激素。雄性 ICR 小鼠（9 周龄）注射 DEX（40mg/kg），并连续 14 天每天口服 SCP（30、100 和 300mg/kg）。使用旷场试验（OFT）、蔗糖偏好试验（SPT）、悬尾试验（TST）和强迫游泳试验（FST）确定抑郁样行为。结果表明，SCP 治疗可显著缓解 DEX 诱导的抑郁样行为，并在浓度依赖的方式下减轻 SH-SY5Y 细胞的神经毒性。此外，重复注射 DEX 会显著降低大脑源性神经营养因子（BDNF）、原肌球蛋白受体激酶 B（TrkB）和环磷腺苷反应元件结合蛋白（CREB）的水平，而 SCP 治疗可改善皮质酮诱导的神经毒性。这些结果表明，SCP 在 DEX 诱导的抑郁动物模型中表现出显著的抗抑郁样作用，这种作用可能是通过预防皮质酮诱导的神经毒性介导的。
