Li Ao-Lin, Chen Lü-Jun, Zhang Yan, Dai Tian-Jiao, Tian Jin-Ping, Liu Rui, Wen Dong-Hui
School of Environment, Tsinghua University, Beijing 100084, China.
College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China.
Huan Jing Ke Xue. 2018 Oct 8;39(10):4593-4600. doi: 10.13227/j.hjkx.201801262.
Wastewater treatment plants (WWTPs) have been regarded as important point-sources of antibiotic resistance genes (ARGs) in aquatic environments. To investigate the distribution and removal of ARGs in WWTPs, a pharmaceutical wastewater treatment plant (PWWTP) and an integrated wastewater treatment plant (IWWTP) in a fine-chemical industrial park were chosen, and polymerase chain reaction (PCR) and real-time PCR techniques were used to determine the occurrence and abundances of ARGs along the treatment processes. Ten and fifteen ARGs were detected initially in the influents of PWWTP and IWWTP respectively, in which tetracycline and sulfonamide resistance genes were frequently reported, while was first reported in WWTPs. The most abundant ARGs in the influents were and , followed by , , , , and . The total ARGs increased by 0.21 log after the treatment by PWWTP, whose effluent contributed 0.87% to the inflow yet 5.05% to the total ARGs of IWWTP. Finally the total ARGs removed by IWWTP was 1.03 log, with the remaining ARGs then transported within the final effluent to the nearby coastal area. The authors concluded that the environmental and other impacts from the spread of ARGs on the microbial communities of the coastal environment needed further study.
污水处理厂一直被视为水环境中抗生素抗性基因（ARGs）的重要点源。为了研究污水处理厂中ARGs的分布和去除情况，选取了精细化工产业园区内的一座制药污水处理厂（PWWTP）和一座综合污水处理厂（IWWTP），并采用聚合酶链反应（PCR）和实时荧光定量PCR技术来测定处理过程中ARGs的出现情况和丰度。最初在PWWTP和IWWTP的进水分别检测到10种和15种ARGs，其中四环素和磺胺类抗性基因屡有报道，而[此处原文缺失基因名称]首次在污水处理厂中被报道。进水中最丰富的ARGs是[此处原文缺失基因名称]和[此处原文缺失基因名称]，其次是[此处原文缺失基因名称]、[此处原文缺失基因名称]、[此处原文缺失基因名称]、[此处原文缺失基因名称]和[此处原文缺失基因名称]。PWWTP处理后总ARGs增加了0.21个对数，其出水占进水的0.87%，但占IWWTP总ARGs的5.05%。最后，IWWTP去除的总ARGs为1.03个对数，剩余的ARGs随后随最终出水排入附近沿海地区。作者得出结论，ARGs传播对沿海环境微生物群落的环境及其他影响需要进一步研究。
