Wu Man-Li, Zhu Chang-Cheng, Qi Yan-Yun, Shi Yi-Xin, Xu Hui-Ning, Yang Jin-Ru
Key Laboratory of Environmental Engineering, Shaanxi Province, Key Laboratory of Northwest Water Resources, Environment and Ecology, Ministry of Education, Xi'an University of Architecture and Technology, Xi'an 710055, China.
Huan Jing Ke Xue. 2018 Oct 8;39(10):4802-4808. doi: 10.13227/j.hjkx.201711077.
A 17-estradiol (E2) degrading strain (designated as Wu-SP1) was isolated from the activated sludge collected from a wastewater treatment plant (WWTP) in Xi'an. The strain was identified as sp. according to 18S rDNA sequence and phylogenetic analysis. The optimal pH and temperature for E2 degradation were 6 and 30℃, respectively. Under these conditions, the E2 biodegradation rate of 2 mg·L E2 amounted to 92.5% within 48 h by this strain. The kinetics of E2 degradation by the strain KY123915 were in good accord with the first-order equation, with the concentration ranged from 10 to 500 mg·L. UV spectrum analysis showed the strength of maximum absorption of metabolites became weak compared to E2, indicating that E2 may be degraded via estrone (E1) by sp. KY123915.
从西安某污水处理厂收集的活性污泥中分离出一株17-β-雌二醇（E2）降解菌（命名为Wu-SP1）。根据18S rDNA序列和系统发育分析，该菌株被鉴定为苍白杆菌属。E2降解的最适pH值和温度分别为6和30℃。在此条件下，该菌株对2 mg·L E2的生物降解率在48 h内达到92.5%。菌株KY123915对E2的降解动力学与一级方程吻合良好，浓度范围为10至500 mg·L。紫外光谱分析表明，与E2相比，代谢产物最大吸收强度变弱，表明KY123915菌株可能通过雌酮（E1）降解E2。
