Ren Sheng-Tao, Guo Xia-Li, Lu A-Qian, Zhang Qian-Qian, Guo Xiao-Ying, Wang Yan, Wang Lian-Zhong, Zhang Bao-Bao
College of Chemical Engineering and Energy, Zhengzhou University, Zhengzhou 450001, China.
Henan Xinxiang Huaxing Pharmaceutical Factory, Xinxiang 453731, China.
Huan Jing Ke Xue. 2018 Oct 8;39(10):4817-4824. doi: 10.13227/j.hjkx.201711210.
Aerobic composting experiments were conducted using lincomycin mycelia wastes (dreg) and manure (T), using sewage sludge with manure as a control (CK). High performance liquid phase methods and high throughput sequencing were used to determine the concentration of lincomycin residue and to characterize the microbial community. The results showed that lincomycin was reduced significantly, with the concentration decreasing from 1800 mg·kg to 483 mg·kg, accounting for 73% degradation. In addition, the bacterial community abundance and diversity indices were all lower than that of sludge-manure at the mesophilic and thermophilic phases, because of the high concentration of lincomycin residue in lincomycin mycelia dreg. By contrast, the fungal community abundance and diversity indices showed the reverse, due to the high content of organic matter and nitrogen in lincomycin mycelia dreg. Therefore, the microbial communities were greatly different between T and CK treatment with the domain genera of , , , , , and in T compost and , , , , and in CK compost. However, as the composting continued to a mature phase, most of the lincomycin was reduced, and the differences between the two microbial communities gradually decreased. This showed that composting could make lincomycin mycelia dreg harmless and could be used to turn it into a resource.
以林可霉素菌丝体废渣（菌渣）和猪粪为原料进行好氧堆肥实验（T组），以污泥与猪粪混合为对照（CK组）。采用高效液相法和高通量测序法测定林可霉素残留浓度并对微生物群落进行表征。结果表明，林可霉素显著降低，浓度从1800 mg·kg降至483 mg·kg，降解率达73%。此外，在嗜温阶段和嗜热阶段，由于林可霉素菌渣中林可霉素残留浓度较高，T组细菌群落丰度和多样性指数均低于污泥-猪粪组。相反，由于林可霉素菌渣中有机质和氮含量较高，T组真菌群落丰度和多样性指数则呈现相反趋势。因此，T组和CK组处理之间的微生物群落差异很大，T组堆肥中的优势属为 、 、 、 、 、 ，CK组堆肥中的优势属为 、 、 、 、 。然而，随着堆肥进入腐熟阶段，大部分林可霉素被降解，两个微生物群落之间的差异逐渐减小。这表明堆肥可使林可霉素菌渣无害化，并可将其转化为资源。
