Department of Clinical Pharmacology Lab, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, P. R. China.
The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210046, P. R. China.
Biomater Sci. 2018 Nov 1;6(11):2925-2931. doi: 10.1039/c8bm00399h. Epub 2018 Sep 19.
Owing to the unique advantages of high specificity and minimal invasiveness, photothermal therapy (PTT) has been evidenced with great potential in cancer treatment. However, most photothermal agents present a shortage of photobleaching and nonspecific biodistribution in clinical application. In this study, we conjugated a new Indocyanine Green Dye (IR820) with self-assembled polypeptide (ELP) via chemical bonding in an aqueous environment. This preparation method could effectively avoid damaging the polypeptide. ELP-IR820 was fabricated as nanoconjugates with diameters of approximately 50 nm. The use of ELP-IR820 notably enhanced photothermal-mediated cytotoxicity on CT-26 cancer cells. We demonstrate that the ELP-IR820 nanoparticles significantly improved drug accumulation in the tumor and photothermal effect in vivo compared to the free dye and monomer ELP-IR820. ELP-IR820 nanoparticle also exhibited outstanding ability to cause prominent tumor tissue growth inhibition via the photothermal effect. No noticeable toxicity was detected for all treatment groups. These investigations broaden the application of NIR dyes as a multimodal photothermal therapy platform.
由于光热疗法（PTT）具有特异性高和微创性等独特优势，在癌症治疗方面具有很大的潜力。然而，大多数光热试剂在临床应用中存在光漂白和非特异性生物分布的不足。在本研究中，我们通过在水相环境中化学结合将一种新型吲哚菁绿染料（IR820）与自组装多肽（ELP）偶联。这种制备方法可以有效地避免破坏多肽。ELP-IR820 被制成直径约为 50nm 的纳米复合物。使用 ELP-IR820 显著增强了 CT-26 癌细胞的光热介导细胞毒性。我们证明，与游离染料和单体 ELP-IR820 相比，ELP-IR820 纳米颗粒显著增加了肿瘤内的药物积累和体内的光热效应。ELP-IR820 纳米颗粒还通过光热效应表现出显著的抑制肿瘤组织生长的能力。所有治疗组均未检测到明显的毒性。这些研究拓宽了近红外染料作为多模态光热治疗平台的应用。
