Venkatasubramanian P N, Arús C, Bárány M
Clin Physiol Biochem. 1986;4(5):285-92.
In continuation of our previous work on one-dimensional (1D) proton nuclear magnetic resonance (1H-NMR) of normal and diseased human muscle extracts we recorded the two-dimensional (2D) J-correlated proton magnetic resonance spectra of these extracts. Significant differences between normal and diseased muscle extracts, not observed in the 1D 1H-NMR spectra, were seen from their 2D connectivity contour patterns. Taurine was not present in cerebral palsy muscle extract while both normal and scoliosis muscles contained this metabolite. Only the normal muscle had carnitine. Carnosine was present in all muscles. alpha-Ketoglutarate was found only in the diseased muscle extracts. While the amino acids lysine, cysteine and glutamine were common to normal and diseased muscles, threonine was seen only in the diseased muscles. Additional small differences were detected in the 2D patterns of human muscle extracts.
在我们之前关于正常和患病人类肌肉提取物的一维（1D）质子核磁共振（1H-NMR）研究的基础上，我们记录了这些提取物的二维（2D）J-相关质子磁共振谱。从它们的二维连接轮廓模式中可以看出，正常和患病肌肉提取物之间存在显著差异，而这些差异在一维1H-NMR谱中并未观察到。脑瘫肌肉提取物中不存在牛磺酸，而正常和脊柱侧弯肌肉中都含有这种代谢物。只有正常肌肉含有肉碱。肌肽存在于所有肌肉中。α-酮戊二酸仅在患病肌肉提取物中被发现。虽然赖氨酸、半胱氨酸和谷氨酰胺这几种氨基酸在正常和患病肌肉中都存在，但苏氨酸仅在患病肌肉中出现。在人类肌肉提取物的二维模式中还检测到了其他细微差异。
