Arús C, Bárány M, Westler W M, Markley J L
Clin Physiol Biochem. 1984;2(1):49-55.
High resolution proton nuclear magnetic resonance (NMR) spectra of normal and diseased human muscle extracts were recorded at 470 MHz. Resonances from lactic acid, creatine, glucose, ribose, purine and pyrimidine bases were identified. The longitudinal relaxation times of these resonances were determined to allow quantitation of muscle metabolites. With aid of a standardized reference capillary, inserted into the NMR tube containing the muscle extracts, the lactic acid and total creatine content of the extracts was determined. After 5 h of incubation at 37 degrees C, normal muscles contained on average 103 mumol lactic acid and 36 mumol creatine/173 mg of noncollagenous protein, equivalent to 1.0 g of fresh muscle. The lactic acid and creatine contents decreased slightly in scoliosis and idiopathic scoliosis and they decreased significantly in cerebral palsy. In an extract of a patient whose illness was diagnosed as 'scoliosis' no creatine was present, and in an extract of a patient with unknown diagnosis the creatine content was reduced to 2 mumol/173 mg of noncollagenous protein. The short time (1.7 sec to 6.5 min) and the small amount of tissue (300 mg) needed for an analysis add to the potential of proton NMR as a new technique for the characterization of muscular diseases.
在470兆赫下记录了正常和患病人类肌肉提取物的高分辨率质子核磁共振（NMR）光谱。鉴定出来自乳酸、肌酸、葡萄糖、核糖、嘌呤和嘧啶碱的共振信号。测定了这些共振信号的纵向弛豫时间，以便对肌肉代谢物进行定量分析。借助插入装有肌肉提取物的核磁共振管中的标准化参考毛细管，测定了提取物中乳酸和总肌酸的含量。在37摄氏度孵育5小时后，正常肌肉平均含有103微摩尔乳酸和36微摩尔肌酸/173毫克非胶原蛋白，相当于1.0克新鲜肌肉。乳酸和肌酸含量在脊柱侧凸和特发性脊柱侧凸中略有下降，而在脑瘫中则显著下降。在一名被诊断为“脊柱侧凸”患者的提取物中没有肌酸，在一名诊断不明患者的提取物中，肌酸含量降至2微摩尔/173毫克非胶原蛋白。分析所需的短时间（1.7秒至6.5分钟）和少量组织（300毫克）增加了质子核磁共振作为一种表征肌肉疾病的新技术的潜力。
