Department of Spine Surgery, The Third Hospital of Hebei Medical University, Shijiazhuang, China.
Eur Rev Med Pharmacol Sci. 2018 Sep;22(17):5416-5423. doi: 10.26355/eurrev_201809_15800.
We investigate whether microRNA-27a-3p (miR-27a-3p) can inhibit the inflammatory response of spinal cord injury by negatively regulating toll-like receptor 4 (TLR4).
The quantitative Real-time polymerase chain reaction (qRT-PCR) assay was used to detect the expression of miR-27a-3p and TLR4 in serum samples from patients with spinal cord injury and in hydrogen peroxide-treated C8-B4 and C8-D1A cells. Dual luciferase reporter assays were used to detect targeted binding of TLR4 to miR-27a-3p. The protein expression of miR-27a-3p and TLR4 and the two inflammatory factors, tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6), were all detected by Western blot.
TLR4 expression was elevated and miR-27a-3p was decreased in serum samples from patients with spinal cord injury and in hydrogen peroxide-treated C8-D1A and C8-B4 cells. Dual luciferase reporter assays results demonstrated that miR-27a-3p can bind to TLR4. Up-regulation of miR-27a-3p can decrease the expression of TNF-α and IL-6 and can also reduce TLR4 expression. After overexpression of TLR4, the expression of TNF-α and IL-6 were increased.
miR-27a-3p can inhibit the inflammatory response of spinal cord injury by negatively regulating TLR4.
通过负调控 Toll 样受体 4（TLR4），研究 microRNA-27a-3p（miR-27a-3p）是否可以抑制脊髓损伤的炎症反应。
采用实时定量聚合酶链反应（qRT-PCR）检测脊髓损伤患者血清中 miR-27a-3p 和 TLR4 的表达，并用过氧化氢处理 C8-B4 和 C8-D1A 细胞。采用双荧光素酶报告基因实验检测 TLR4 与 miR-27a-3p 的靶向结合。采用 Western blot 检测 miR-27a-3p 和 TLR4 的蛋白表达以及两种炎症因子肿瘤坏死因子-α（TNF-α）和白细胞介素 6（IL-6）。
脊髓损伤患者血清样本以及过氧化氢处理的 C8-D1A 和 C8-B4 细胞中 TLR4 表达升高，miR-27a-3p 表达降低。双荧光素酶报告基因实验结果表明，miR-27a-3p 可与 TLR4 结合。上调 miR-27a-3p 可降低 TNF-α和 IL-6 的表达，并降低 TLR4 表达。过表达 TLR4 后，TNF-α和 IL-6 的表达增加。
miR-27a-3p 通过负调控 TLR4 抑制脊髓损伤的炎症反应。
