Kim Ha Yeong, Jung Soo Yeon, Lee Sang Jin, Lee Hyun Jung, Truong Minh-Dung, Kim Han Su
Department of Molecular Medicine, Ewha Womans University, Seoul.
Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Ewha Womans University, Seoul.
Laryngoscope. 2019 Feb;129(2):351-357. doi: 10.1002/lary.27344. Epub 2018 Sep 19.
Aesthetic reconstruction of the external ear is challenging due to the complex anatomical shape of the auricle. Recently, artificial scaffolds such as Medpor (Stryker, Kalamasoo, MI, USA) have become widely used in ear reconstruction. However, the Medpor scaffold is stiffer than the natural ear, which may lead to discomfort, and moreover has uniform design for every patient. In this study, we investigated whether three-dimensional (3D)-printed artificial polyurethane (PU) scaffolds are suitable for auricular reconstruction.
PU scaffolds were fabricated using 3D printing according to a design derived from a digital imaging and communications in medicine (DICOM) image of the human auricle. The microstructure of the scaffolds was observed using scanning electron microscopy, and the porosity was examined. Cell proliferation on the scaffolds was assessed in vitro using tonsil-derived mesenchymal stem cells to evaluate the biocompatibility of the scaffolds. The scaffolds were implanted in C57BL/6 mice, and histological analysis was performed.
The structural study revealed that the 3D-printed porous PU scaffolds have rectangular microstructure with regular pitch and line, as well as high porosity (56.46% ± 10.22%) with a pore diameter of 200 µm. The mechanical properties of the 3D-printed PU scaffolds were similar to those of the human auricle cartilage. Cell proliferation on the PU scaffolds was greater than that on Medpor scaffolds. Histological evaluation demonstrated that the porous parts of the PU scaffolds became filled with collagen and vascular tissue.
Elastic, porous PU scaffolds can be obtained using 3D printing, have biomechanical properties similar to those of the natural ear, and are suitable for use in auricular reconstruction.
NA Laryngoscope, 129:351-357, 2019.
由于耳廓复杂的解剖形状，外耳的美学重建具有挑战性。近年来，诸如Medpor（美国密歇根州卡拉马祖市史赛克公司）等人工支架已广泛应用于耳廓重建。然而，Medpor支架比天然耳朵更硬，这可能导致不适，而且对每个患者的设计都是统一的。在本研究中，我们调查了三维（3D）打印的人工聚氨酯（PU）支架是否适用于耳廓重建。
根据从人耳廓的数字成像和通信医学（DICOM）图像得出的设计，使用3D打印制造PU支架。使用扫描电子显微镜观察支架的微观结构，并检查孔隙率。使用扁桃体来源的间充质干细胞在体外评估支架上的细胞增殖，以评估支架的生物相容性。将支架植入C57BL/6小鼠体内，并进行组织学分析。
结构研究表明，3D打印的多孔PU支架具有矩形微观结构，间距和线条规则，孔隙率高（56.46%±10.22%），孔径为200µm。3D打印的PU支架的力学性能与人耳廓软骨相似。PU支架上的细胞增殖大于Medpor支架上的细胞增殖。组织学评估表明，PU支架的多孔部分充满了胶原蛋白和血管组织。
使用3D打印可获得具有弹性的多孔PU支架，其生物力学性能与天然耳朵相似，适用于耳廓重建。
无 喉镜，2019年，第129卷，第351 - 357页
