Ismail-Beigi F, Haber R S, Loeb J N
Endocrinology. 1986 Dec;119(6):2527-36. doi: 10.1210/endo-119-6-2527.
A continuous cell line derived from rat liver (ARL 15) has been identified that responds to thyroid hormone with a stimulation of active Na,K transport. Stimulation of ouabain-inhibitable K+ uptake, which is half-maximal at a T3 concentration of 1.4 X 10(-10) M, is accompanied by corresponding increases in passive Na+ influx and in passive K+ efflux. The enhancement of Na+ and K+ fluxes by T3 is shown to be accompanied by smaller and equivalent increases both in enzymatically measured Na,K-ATPase activity and in maximal ouabain-sensitive Na,K transport in the presence of the Na+ ionophore monensin. The demonstration that both passive Na+ influx and passive fractional K+ efflux are simultaneously increased by T3 supports the earlier suggestion that the stimulation of active Na,K transport by thyroid hormone is attributable, at least in part, to an enhancement of rate-limiting passive Na+ and K+ fluxes by an increase in membrane permeability.
已鉴定出一种源自大鼠肝脏的连续细胞系（ARL 15），它对甲状腺激素有反应，可刺激主动钠钾转运。哇巴因抑制的钾摄取受到刺激，在三碘甲状腺原氨酸（T3）浓度为1.4×10⁻¹⁰ M时达到半最大刺激，同时伴随着被动钠内流和被动钾外流相应增加。T3对钠和钾通量的增强伴随着在存在钠离子载体莫能菌素的情况下，通过酶促测定的钠钾ATP酶活性以及最大哇巴因敏感钠钾转运的较小且相当的增加。T3同时增加被动钠内流和被动钾外流分数的证明支持了早期的观点，即甲状腺激素对主动钠钾转运的刺激至少部分归因于通过增加膜通透性来增强限速被动钠和钾通量。
