Gebhardt R, Schäfer-Degenhart I
Physiologisch-chemisches Institut Universität Tubingen, Federal Republic of Germany.
Cell Biol Toxicol. 1988 Dec;4(4):379-92. doi: 10.1007/BF00117767.
Monoclonal antibodies directed against antigens on rat liver epithelial cell lines were prepared. Three antibodies, 4C3, 19C6, and 3C2, recognized surface antigens present (although in different quantities) on eight epithelial cell lines tested, irrespective of whether they were normal or transformed. For MAb 3C2, the primary antigen common to all but one cell line showed a Mr of 135 kD. In paraffin sections of liver tissue, two antibodies, 4C3 and 19C6, reacted exclusively with bile duct epithelium, whereas the MAb 3C2 additionally reacted with sinusoidal endothelium and the endothelium of the portal venules. In sections of livers from rats exposed to diethylnitrosamine, the MAb 19C6 selectively stained bile duct-like structures in cholangiomas, while other preneoplastic and neoplastic lesions were not stained. These results demonstrate that the monoclonal antibodies obtained may prove useful for investigating cell lineages related to propagable liver epithelial cell lines and suggest that these cells may be derived from terminal bile ductular cells.
制备了针对大鼠肝上皮细胞系抗原的单克隆抗体。三种抗体，即4C3、19C6和3C2，识别所测试的八种上皮细胞系上存在的表面抗原（尽管数量不同），无论它们是正常的还是转化的。对于单克隆抗体3C2，除一个细胞系外所有细胞系共有的主要抗原的分子量为135 kD。在肝组织石蜡切片中，两种抗体4C3和19C6仅与胆管上皮反应，而单克隆抗体3C2还与窦状内皮和门静脉小静脉内皮反应。在暴露于二乙基亚硝胺的大鼠肝脏切片中，单克隆抗体19C6选择性地染色胆管瘤中的胆管样结构，而其他癌前和肿瘤性病变未被染色。这些结果表明，所获得的单克隆抗体可能对研究与可增殖肝上皮细胞系相关的细胞谱系有用，并表明这些细胞可能源自终末胆小管细胞。
