Kawamoto F, Fujioka H, Kumada N, Kojima K
Exp Parasitol. 1986 Dec;62(3):309-15. doi: 10.1016/0014-4894(86)90036-6.
A mode of action of the inducible treatment with trypsin for the development of Mesocestoides lineatus tetrathyridium to adult was analyzed by administering various agents effective on Ca2+-dependent metabolic pathways in the cells: protein kinase C activators such as a synthetic diacylglycerol, 1-oleoyl-2-acetylglycerol, and a tumor promoting phorbol, 12-O-tetra-decanoyl-phorbol-13-acetate, enhanced the trypsin induced developmental processes. On the contrary, a calmodulin inhibitor, N-(6-aminohexyl)-5-chloro-1-naphthalene sulfonamide, cyclic adenosine 3',5'-monophosphate, and adenylate cyclase activators such as forskolin and cholera toxin, inhibited the triggering action of trypsin. Furthermore, a combined administration of Ca2+ ionophore (A23187) and the phorbol showed a similar effect with trypsin treatment, and sodium taurocholate acted as a potent enhancer like the activators of protein kinase C. These results strongly suggest that the initiation of development to adult in this cestode may be regulated synergistically by Ca2+ and protein kinase C, and that a bile acid may be involved in an activation mechanism of protein kinase C.
通过给予对细胞中钙依赖性代谢途径有效的各种试剂，分析了用胰蛋白酶进行诱导处理以使线形中绦虫四槽蚴发育为成虫的作用模式：蛋白激酶C激活剂，如合成二酰基甘油、1-油酰基-2-乙酰甘油，以及促肿瘤佛波醇、12-O-十四烷酰佛波醇-13-乙酸酯，增强了胰蛋白酶诱导的发育过程。相反，钙调蛋白抑制剂N-(6-氨基己基)-5-氯-1-萘磺酰胺、环腺苷3',5'-单磷酸，以及腺苷酸环化酶激活剂，如福斯高林和霍乱毒素，抑制了胰蛋白酶的触发作用。此外，联合给予钙离子载体(A23187)和佛波醇显示出与胰蛋白酶处理相似的效果，牛磺胆酸钠像蛋白激酶C激活剂一样起到了强效增强剂的作用。这些结果有力地表明，这种绦虫发育为成虫的起始过程可能受钙离子和蛋白激酶C协同调节，并且胆汁酸可能参与蛋白激酶C的激活机制。
