Calcium-sensitive pyruvate dehydrogenase phosphatase is required for energy metabolism, growth, differentiation, and infectivity of .

In vertebrate cells, mitochondrial Ca uptake by the mitochondrial calcium uniporter (MCU) leads to Ca-mediated stimulation of an intramitochondrial pyruvate dehydrogenase phosphatase (PDP). This enzyme dephosphorylates serine residues in the E1α subunit of pyruvate dehydrogenase (PDH), thereby activating PDH and resulting in increased ATP production. Although a phosphorylation/dephosphorylation cycle for the E1α subunit of PDH from nonvertebrate organisms has been described, the Ca-mediated PDP activation has not been studied. In this work, we investigated the Ca sensitivity of two recombinant PDPs from the protozoan human parasites (TcPDP) and (TbPDP) and generated a -KO cell line to establish TcPDP's role in cell bioenergetics and survival. Moreover, the mitochondrial localization of the TcPDP was studied by CRISPR/Cas9-mediated endogenous tagging. Our results indicate that TcPDP and TbPDP both are Ca-sensitive phosphatases. Of note, -KO epimastigotes exhibited increased levels of phosphorylated TcPDH, slower growth and lower oxygen consumption rates than control cells, an increased AMP/ATP ratio and autophagy under starvation conditions, and reduced differentiation into infective metacyclic forms. Furthermore, -KO trypomastigotes were impaired in infecting cultured host cells. We conclude that TcPDP is a Ca-stimulated mitochondrial phosphatase that dephosphorylates TcPDH and is required for normal growth, differentiation, infectivity, and energy metabolism in Our results support the view that one of the main roles of the MCU is linked to the regulation of intramitochondrial dehydrogenases.