Abarca Juan G, Vargas Gabriel, Zuniga Ibrahim, Whitfield Steven M, Woodhams Douglas C, Kerby Jacob, McKenzie Valerie J, Murillo-Cruz Catalina, Pinto-Tomás Adrián A
Centro de Investigación en Estructuras Microscópicas, Universidad de Costa Rica, San Pedro, Costa Rica.
Department of the Geophysical Sciences, University of Chicago, Chicago, IL, United States.
Front Microbiol. 2018 Sep 3;9:2001. doi: 10.3389/fmicb.2018.02001. eCollection 2018.
Amphibian skin is a suitable environment for rich communities of microorganisms, both beneficial and detrimental to the host. The amphibian cutaneous microbiota has been hypothesized to play an important role as symbionts, protecting their hosts against disease. Costa Rica has one of the most diverse assemblages of amphibians in the world and we know very little about the microbiota of these tropical animals. For comparison with other studies, we explore the diversity of the skin bacterial communities employing16S rRNA amplicon sequencing of swab samples from twelve species of frogs at La Selva Biological Station in Sarapiquí, Heredia province. The predominant phylum detected in our studies was Proteobacteria, followed by Bacteroidetes and Actinobacteria, with these three phyla representing 89.9% of the total bacterial taxa. At the family level, Sphingobacteriaceae and Comamonadaceae were highly represented among samples. Our results suggest that host species and host family are significant predictors of the variation in microbiota composition. This study helps set the foundation for future research about microbiota composition and resilience to unfavorable conditions, leading to improvement in managing strategies for endangered amphibian species.
两栖动物的皮肤是微生物丰富群落的适宜生存环境,这些微生物对宿主既有益处也有损害。两栖动物的皮肤微生物群被认为作为共生体发挥着重要作用,保护其宿主免受疾病侵害。哥斯达黎加拥有世界上最多样化的两栖动物种群之一,但我们对这些热带动物的微生物群了解甚少。为了与其他研究进行比较,我们采用16S rRNA扩增子测序技术,对来自埃雷迪亚省萨拉皮卡的拉塞尔瓦生物站的12种青蛙的拭子样本进行分析,以探究皮肤细菌群落的多样性。在我们的研究中检测到的主要菌门是变形菌门,其次是拟杆菌门和放线菌门,这三个菌门占细菌总分类群的89.9%。在科的水平上,鞘脂杆菌科和丛毛单胞菌科在样本中占比很高。我们的结果表明,宿主物种和宿主科是微生物群组成变化的重要预测因素。这项研究有助于为未来关于微生物群组成及其对不利条件的恢复力的研究奠定基础,从而改进濒危两栖动物物种的管理策略。