• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

细胞毒性坏死因子 1 通过下调巨噬细胞中 CD36 的转录来诱导急性尿路感染期间的炎症反应。

Cytotoxic Necrotizing Factor 1 Downregulates CD36 Transcription in Macrophages to Induce Inflammation During Acute Urinary Tract Infections.

机构信息

Key Laboratory of Immune Microenvironment and Disease of the Educational Ministry of China, Tianjin Key Laboratory of Cellular and Molecular Immunology, Department of Immunology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China.

State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Molecular Drug Research, Collaborative Innovation Center for Biotherapy, College of Pharmacy, Nankai University, Tianjin, China.

出版信息

Front Immunol. 2018 Aug 31;9:1987. doi: 10.3389/fimmu.2018.01987. eCollection 2018.

DOI:10.3389/fimmu.2018.01987
PMID:30233583
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6128224/
Abstract

Urinary tract infections (UTIs) caused by uropathogenic (UPEC) induce cystitis, pyelonephritis, and can cause kidney scarring and failure if inflammation is not under control. The detailed effects of cytotoxic necrotizing factor 1 (CNF1), the key UPEC toxin, on the pathogenicity of UPEC remain unclear. CD36 is an important scavenger receptor, responsible for pathogen and apoptotic cell clearance, and plays an essential role in host immune defense and homeostasis. Regulation of CD36 by bacterial toxins has not been reported. In this study, using a pyelonephritis mouse model, CNF1 was observed to contribute to increasing neutrophils and bacterial titers in infected bladder and kidney tissues, resulting in severe inflammation and tissue damage. CD36 expression in macrophages was found to be decreased by CNF1 and . We demonstrated that CNF1 attenuated CD36 transcription by decreasing expressions of its upstream transcription factors LXRβ and C/EBPα and their recruitment to the CD36 promotor. In addition, Cdc42 was found to be involved in CNF1-mediated downregulation of LXRβ. Our study investigated the pathogenesis of -carrying UPEC, which affected host innate immune defenses and homeostasis through regulation of CD36 in macrophages during acute UTIs.

摘要

尿路致病性大肠杆菌 (UPEC) 引起的尿路感染会导致膀胱炎、肾盂肾炎，如果炎症得不到控制，还会导致肾脏瘢痕和衰竭。细胞毒性坏死因子 1 (CNF1) 作为 UPEC 的关键毒素，其对 UPEC 致病性的详细影响仍不清楚。CD36 是一种重要的清道夫受体，负责清除病原体和凋亡细胞，在宿主免疫防御和内稳态中发挥重要作用。细菌毒素对 CD36 的调节尚未见报道。在这项研究中，我们使用肾盂肾炎小鼠模型发现，CNF1 有助于增加感染膀胱和肾脏组织中的中性粒细胞和细菌滴度，导致严重的炎症和组织损伤。研究发现 CNF1 下调巨噬细胞中 CD36 的表达。我们证明 CNF1 通过降低其上游转录因子 LXRβ 和 C/EBPα 的表达及其与 CD36 启动子的募集，从而减弱 CD36 的转录。此外，还发现 Cdc42 参与了 CNF1 介导的 LXRβ 下调。我们的研究调查了携带 -的 UPEC 的发病机制，该机制通过在急性尿路感染期间调节巨噬细胞中的 CD36 影响宿主固有免疫防御和内稳态。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44d3/6128224/aa8134d74b87/fimmu-09-01987-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44d3/6128224/f792f3d7ed51/fimmu-09-01987-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44d3/6128224/9e255c5f52a4/fimmu-09-01987-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44d3/6128224/63a416d0ed44/fimmu-09-01987-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44d3/6128224/95d1c15f1a45/fimmu-09-01987-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44d3/6128224/228e8e7ca5d1/fimmu-09-01987-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44d3/6128224/c5c084c30aff/fimmu-09-01987-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44d3/6128224/e7c9a55d3f62/fimmu-09-01987-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44d3/6128224/aa8134d74b87/fimmu-09-01987-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44d3/6128224/f792f3d7ed51/fimmu-09-01987-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44d3/6128224/9e255c5f52a4/fimmu-09-01987-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44d3/6128224/63a416d0ed44/fimmu-09-01987-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44d3/6128224/95d1c15f1a45/fimmu-09-01987-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44d3/6128224/228e8e7ca5d1/fimmu-09-01987-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44d3/6128224/c5c084c30aff/fimmu-09-01987-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44d3/6128224/e7c9a55d3f62/fimmu-09-01987-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44d3/6128224/aa8134d74b87/fimmu-09-01987-g0008.jpg

相似文献

1
Cytotoxic Necrotizing Factor 1 Downregulates CD36 Transcription in Macrophages to Induce Inflammation During Acute Urinary Tract Infections.细胞毒性坏死因子 1 通过下调巨噬细胞中 CD36 的转录来诱导急性尿路感染期间的炎症反应。
Front Immunol. 2018 Aug 31;9:1987. doi: 10.3389/fimmu.2018.01987. eCollection 2018.
2
Cytotoxic Necrotizing Factor-1 (CNF1) does not promote E. coli infection in a murine model of ascending pyelonephritis.细胞毒性坏死因子-1（CNF1）在小鼠上行性肾盂肾炎模型中不会促进大肠杆菌感染。
BMC Microbiol. 2017 May 25;17(1):127. doi: 10.1186/s12866-017-1036-0.
3
Antibodies against hemolysin and cytotoxic necrotizing factor type 1 (CNF1) reduce bladder inflammation in a mouse model of urinary tract infection with toxigenic uropathogenic Escherichia coli.针对溶血素和1型细胞毒性坏死因子（CNF1）的抗体可减轻产毒尿路致病性大肠杆菌引起的小鼠尿路感染模型中的膀胱炎症。
Infect Immun. 2015 Apr;83(4):1661-73. doi: 10.1128/IAI.02848-14. Epub 2015 Feb 9.
4
Cytotoxic necrotizing factor 1 and hemolysin from uropathogenic Escherichia coli elicit different host responses in the murine bladder.尿路致病性大肠埃希菌的细胞毒性坏死因子 1 和溶血素在小鼠膀胱中引发不同的宿主反应。
Infect Immun. 2013 Jan;81(1):99-109. doi: 10.1128/IAI.00605-12. Epub 2012 Oct 22.
5
Mutation of the gene encoding cytotoxic necrotizing factor type 1 (cnf(1)) attenuates the virulence of uropathogenic Escherichia coli.编码细胞毒性坏死因子1（cnf(1)）的基因突变会减弱尿路致病性大肠杆菌的毒力。
Infect Immun. 2001 Jun;69(6):3954-64. doi: 10.1128/IAI.69.6.3954-3964.2001.
6
Interactions of Bacterial Toxin CNF1 and Host JAK1/2 Driven by Liquid-Liquid Phase Separation Enhance Macrophage Polarization.液-液相分离驱动的细菌毒素 CNF1 与宿主 JAK1/2 的相互作用增强巨噬细胞极化。
mBio. 2022 Aug 30;13(4):e0114722. doi: 10.1128/mbio.01147-22. Epub 2022 Jun 29.
7
Hemolysin of uropathogenic Escherichia coli evokes extensive shedding of the uroepithelium and hemorrhage in bladder tissue within the first 24 hours after intraurethral inoculation of mice.尿道致病性大肠杆菌的溶血素在小鼠尿道内接种后的最初24小时内，会引起膀胱组织中尿路上皮的大量脱落和出血。
Infect Immun. 2008 Jul;76(7):2978-90. doi: 10.1128/IAI.00075-08. Epub 2008 Apr 28.
8
Specificity of immunomodulator secretion in urinary samples in response to infection by alpha-hemolysin and CNF1 bearing uropathogenic Escherichia coli.携带α-溶血素和细胞毒性坏死因子1的尿路致病性大肠杆菌感染后尿液样本中免疫调节剂分泌的特异性
Cytokine. 2007 Jan;37(1):22-5. doi: 10.1016/j.cyto.2007.02.016. Epub 2007 Mar 26.
9
Cytotoxic necrotizing factor type 1 delivered by outer membrane vesicles of uropathogenic Escherichia coli attenuates polymorphonuclear leukocyte antimicrobial activity and chemotaxis.由尿路致病性大肠杆菌外膜囊泡递送的1型细胞毒性坏死因子会减弱多形核白细胞的抗菌活性和趋化性。
Infect Immun. 2006 Aug;74(8):4401-8. doi: 10.1128/IAI.00637-06.
10
Cytotoxic necrotizing factor 1 promotes prostate cancer progression through activating the Cdc42-PAK1 axis.细胞毒性坏死因子1通过激活Cdc42-PAK1轴促进前列腺癌进展。
J Pathol. 2017 Oct;243(2):208-219. doi: 10.1002/path.4940. Epub 2017 Aug 29.

引用本文的文献

1
Detection of Cyclomodulin CNF-1 Toxin-Producing Strains of in Pig Kidneys at a Slaughterhouse.在一家屠宰场的猪肾脏中检测产环调节蛋白CNF-1毒素的菌株。
Microorganisms. 2023 Aug 11;11(8):2065. doi: 10.3390/microorganisms11082065.
2
Interactions of Bacterial Toxin CNF1 and Host JAK1/2 Driven by Liquid-Liquid Phase Separation Enhance Macrophage Polarization.液-液相分离驱动的细菌毒素 CNF1 与宿主 JAK1/2 的相互作用增强巨噬细胞极化。
mBio. 2022 Aug 30;13(4):e0114722. doi: 10.1128/mbio.01147-22. Epub 2022 Jun 29.
3
Immunomodulatory properties of CNF1 toxin from : implications for colorectal carcinogenesis.

本文引用的文献

1
Temporal Regulation of Genes in Uropathogenic during Infection of the Murine Urinary Tract.尿道致病性大肠杆菌感染小鼠尿道期间基因的时间调控
J Pathog. 2017;2017:8694356. doi: 10.1155/2017/8694356. Epub 2017 Dec 27.
2
Cytotoxic necrotizing factor 1 promotes prostate cancer progression through activating the Cdc42-PAK1 axis.细胞毒性坏死因子1通过激活Cdc42-PAK1轴促进前列腺癌进展。
J Pathol. 2017 Oct;243(2):208-219. doi: 10.1002/path.4940. Epub 2017 Aug 29.
3
Cytotoxic Necrotizing Factor-1 (CNF1) does not promote E. coli infection in a murine model of ascending pyelonephritis.
来自的CNF1毒素的免疫调节特性：对结直肠癌发生的影响
Am J Cancer Res. 2022 Feb 15;12(2):651-660. eCollection 2022.
4
Nucleotide receptors mediate protection against neonatal sepsis and meningitis caused by alpha-hemolysin expressing Escherichia coli K1.核苷酸受体介导针对产α-溶素大肠埃希菌 K1 引起的新生儿败血症和脑膜炎的保护作用。
FASEB J. 2022 Mar;36(3):e22197. doi: 10.1096/fj.202101485R.
5
The Cytotoxic Necrotizing Factors (CNFs)-A Family of Rho GTPase-Activating Bacterial Exotoxins.细胞毒性坏死因子（CNFs）-一类 Rho GTPase 激活细菌外毒素。
Toxins (Basel). 2021 Dec 15;13(12):901. doi: 10.3390/toxins13120901.
6
Effects of the Bacterial Toxin Cytotoxic Necrotizing Factor 1 on Different Human and Animal Cells: A Systematic Review.细菌毒素细胞毒性坏死因子 1 对不同人类和动物细胞的影响：系统评价。
Int J Mol Sci. 2021 Nov 22;22(22):12610. doi: 10.3390/ijms222212610.
7
Oxalate Alters Cellular Bioenergetics, Redox Homeostasis, Antibacterial Response, and Immune Response in Macrophages.草酸盐会改变巨噬细胞的细胞生物能量、氧化还原稳态、抗菌反应和免疫反应。
Front Immunol. 2021 Oct 21;12:694865. doi: 10.3389/fimmu.2021.694865. eCollection 2021.
8
Protein Ligands in the Secretome of CD36 Fibroblasts Induce Growth Suppression in a Subset of Breast Cancer Cell Lines.CD36成纤维细胞分泌组中的蛋白质配体在一部分乳腺癌细胞系中诱导生长抑制。
Cancers (Basel). 2021 Sep 8;13(18):4521. doi: 10.3390/cancers13184521.
9
An infection-induced RhoB-Beclin 1-Hsp90 complex enhances clearance of uropathogenic Escherichia coli.感染诱导的 RhoB-Beclin 1-Hsp90 复合物增强尿路致病性大肠杆菌的清除。
Nat Commun. 2021 May 10;12(1):2587. doi: 10.1038/s41467-021-22726-8.
10
Alpha-hemolysin of uropathogenic Escherichia coli induces GM-CSF-mediated acute kidney injury.产脲原体大肠埃希菌的α-溶血素诱导 GM-CSF 介导的急性肾损伤。
Mucosal Immunol. 2020 Jan;13(1):22-33. doi: 10.1038/s41385-019-0225-6. Epub 2019 Nov 12.
细胞毒性坏死因子-1（CNF1）在小鼠上行性肾盂肾炎模型中不会促进大肠杆菌感染。
BMC Microbiol. 2017 May 25;17(1):127. doi: 10.1186/s12866-017-1036-0.
4
Transcriptional Control of Dual Transporters Involved in α-Ketoglutarate Utilization Reveals Their Distinct Roles in Uropathogenic .参与α-酮戊二酸利用的双重转运体的转录调控揭示了它们在尿路致病性中的不同作用。
Front Microbiol. 2017 Feb 21;8:275. doi: 10.3389/fmicb.2017.00275. eCollection 2017.
5
Distinct gene regulatory programs define the inhibitory effects of liver X receptors and PPARG on cancer cell proliferation.不同的基因调控程序决定了肝脏X受体和PPARG对癌细胞增殖的抑制作用。
Genome Med. 2016 Jul 11;8(1):74. doi: 10.1186/s13073-016-0328-6.
6
CD36 Is a Matrix Metalloproteinase-9 Substrate That Stimulates Neutrophil Apoptosis and Removal During Cardiac Remodeling.CD36是一种基质金属蛋白酶-9底物，在心脏重塑过程中刺激中性粒细胞凋亡和清除。
Circ Cardiovasc Genet. 2016 Feb;9(1):14-25. doi: 10.1161/CIRCGENETICS.115.001249. Epub 2015 Nov 17.
7
A Murine Model for Escherichia coli Urinary Tract Infection.大肠杆菌尿路感染的小鼠模型
Methods Mol Biol. 2016;1333:159-75. doi: 10.1007/978-1-4939-2854-5_14.
8
Uropathogenic Escherichia coli Engages CD14-Dependent Signaling to Enable Bladder-Macrophage-Dependent Control of Acute Urinary Tract Infection.尿路致病性大肠杆菌通过 CD14 依赖性信号通路与膀胱巨噬细胞相互作用，从而实现对急性尿路感染的调控。
J Infect Dis. 2016 Feb 15;213(4):659-68. doi: 10.1093/infdis/jiv424. Epub 2015 Aug 30.
9
CD36 Is Essential for Regulation of the Host Innate Response to Staphylococcus aureus α-Toxin-Mediated Dermonecrosis.CD36对于宿主对金黄色葡萄球菌α毒素介导的皮肤坏死的固有反应调节至关重要。
J Immunol. 2015 Sep 1;195(5):2294-302. doi: 10.4049/jimmunol.1500500. Epub 2015 Jul 29.
10
Macrophages Subvert Adaptive Immunity to Urinary Tract Infection.巨噬细胞破坏对尿路感染的适应性免疫。
PLoS Pathog. 2015 Jul 16;11(7):e1005044. doi: 10.1371/journal.ppat.1005044. eCollection 2015 Jul.