Herrera-Vázquez Arturo, Arellano-Aranda Rebeca, Hernández-Cueto Daniel, Rodríguez-Miranda Esmeralda, López-Briones Sergio, Hernández-Luna Marco Antonio
Department of Medicine and Nutrition, Division of Health Sciences, University of Guanajuato, Campus León, Guanajuato 37670, Mexico.
Department of Veterinary, Division of Life Sciences, University of Guanajuato, Campus Irapuato Salamanca, Guanajuato 36500, Mexico.
Microorganisms. 2023 Aug 11;11(8):2065. doi: 10.3390/microorganisms11082065.
Food is often contaminated with bacteria strains, which have been associated with different diseases, including urinary tract infections. The consumption of meat by humans is a potential route of transmission of antimicrobial resistance, and food-producing animals have been associated as a major reservoir of resistant bacterial strains. The aim of this study was to determine the presence of the strains producing the CNF-1 toxin in pig kidneys. Pig kidneys were collected from a Mexican slaughterhouse and classified according to their coloration into reddish kidneys (RK) and yellowish kidneys (YK). A tissue sample from each kidney was processed for histological analysis, the presence of was determined by conventional PCR assay, and the CNF-1 toxin was detected by both conventional PCR and Western blotting. Herein, an inflammatory cell infiltrate was found in all collected kidneys, regardless of macroscopic differences. Surprisingly, and the CNF-1 toxin were detected in all kidney samples. We clearly demonstrate contamination by CNF-1 toxin-producing in pork kidneys from a slaughterhouse, even in those without apparent damage. This suggests that pork may serve as a reservoir for pathogens, representing an important risk to human health.
食物常常被细菌菌株污染，这些菌株与包括尿路感染在内的不同疾病有关。人类食用肉类是抗菌药物耐药性的一个潜在传播途径，而食用动物已被认为是耐药细菌菌株的主要储存库。本研究的目的是确定猪肾脏中产生CNF-1毒素的菌株的存在情况。从墨西哥一家屠宰场收集猪肾脏，并根据其颜色分为微红肾脏（RK）和微黄肾脏（YK）。对每个肾脏的组织样本进行组织学分析，通过常规PCR检测其存在情况，并通过常规PCR和蛋白质印迹法检测CNF-1毒素。在此，在所有收集的肾脏中均发现有炎性细胞浸润，无论其宏观差异如何。令人惊讶的是，在所有肾脏样本中均检测到了该菌株和CNF-1毒素。我们清楚地证明了来自屠宰场的猪肾脏被产生CNF-1毒素的菌株污染，即使是那些没有明显损伤的肾脏。这表明猪肉可能是病原体的储存库，对人类健康构成重要风险。
