Meller Barbara, Kuźnicki Daniel, Arasimowicz-Jelonek Magdalena, Deckert Joanna, Floryszak-Wieczorek Jolanta
Department of Plant Physiology, Poznań University of Life Sciences, Poznań, Poland.
Department of Plant Ecophysiology, Faculty of Biology, Adam Mickiewicz University in Poznań, Poznań, Poland.
Front Plant Sci. 2018 Aug 29;9:1228. doi: 10.3389/fpls.2018.01228. eCollection 2018.
In this paper we analyzed β-aminobutyric acid (BABA)-primed epigenetic adjustment of potato cv. "Sarpo Mira" to . The first stress-free generation of the potato genotype obtained from BABA-primed parent plants via tubers and seeds showed pronounced resistance to the pathogen, which was tuned with the transcriptional memory of SA-responsive genes. During the early priming phase before the triggering stress, we found robust bistable deposition of histone marks (H3K4me2 and H3K27me3) on the (Non-expressor of genes) and the gene (Suppressor of , Inducible), in which transcription antagonized silencing. Switchable chromatin states of these adverse systemic acquired resistance (SAR) regulators probably reprogrammed responsiveness of the and genes and contributed to stress imprinting. The elevated levels of heritable H3K4me2 tag in the absence of transcription on SA-dependent genes in BABA-primed (F) and its vegetative and generative progeny (F) before pathogen challenge provided evidence for the epigenetic mark for intergenerational memory in potato. Moreover, our study revealed that histone acetylation was not critical for maintaining BABA-primed defense information until the plants were triggered with the virulent pathogen when rapid and boosted s gene expression probably required histone acetyltransferase (HAT) activity both in F and F progeny.
在本文中，我们分析了β-氨基丁酸（BABA）引发的马铃薯品种“Sarpo Mira”的表观遗传调控，以应对……。从经BABA引发处理的亲本植株通过块茎和种子获得的第一代无胁迫马铃薯基因型对病原体表现出显著抗性，这与水杨酸（SA）响应基因的转录记忆相关。在触发胁迫之前的早期引发阶段，我们发现组蛋白标记（H3K4me2和H3K27me3）在（基因的非表达子）和基因（病程相关基因抑制子，可诱导型）上有强大的双稳态沉积，其中转录拮抗沉默。这些负面系统获得性抗性（SAR）调节因子的可切换染色质状态可能重新编程了和基因的反应性，并有助于胁迫印记。在病原体攻击之前，经BABA引发处理的（F）及其营养和生殖后代（F）中，SA依赖性基因在无转录情况下可遗传的H3K4me2标签水平升高，为马铃薯代际记忆的表观遗传标记提供了证据。此外，我们的研究表明，在植物被致病病原体触发之前，组蛋白乙酰化对于维持BABA引发的防御信息并不关键，此时F和F后代中快速且增强的病程相关基因表达可能需要组蛋白乙酰转移酶（HAT）活性。
