Floryszak-Wieczorek Jolanta, Arasimowicz-Jelonek Magdalena, Abramowski Dariusz
Department of Plant Physiology, Poznan University of Life Sciences Poznan, Poland.
Department of Plant Ecophysiology, Faculty of Biology, Adam Mickiewicz University Poznan, Poland.
Front Plant Sci. 2015 Oct 15;6:844. doi: 10.3389/fpls.2015.00844. eCollection 2015.
The transcript of the PR1 gene accumulation as an informative marker of systemic acquired resistance (SAR) was analyzed in β-aminobutyric acid (BABA) primed potato in the short-lasting (3 days) and long-lasting (28 days) time periods after induction and in the vegetative descendants of primed plants derived from tubers and from in vitro seedlings. BABA pretreatment resulted either in minimal or no PR1 gene expression, but sequential treatment with BABA followed by virulent Phytophthora infestans provided data on the imprint of post-stress information and its duration until fertilization, in the form of an enhanced PR1 transcript accumulation and a transient increase of basal resistance to the late blight disease. The primed state for defense of the susceptible potato cultivar was transmitted to its vegetative progeny as a potentiated PR1 mRNA accumulation following challenge inoculation. However, variation was observed between vegetative accessions of the BABA-primed potato genotype in responsiveness to disease. In contrast to plants derived from tubers, potato propagated through in vitro seedlings largely lost inducible resistance traits, although itretained primed PR1 gene expression.
在诱导后的短期（3天）和长期（28天）时间段内，以及在由块茎和离体幼苗衍生的经β-氨基丁酸（BABA）引发处理的马铃薯营养后代中，分析了作为系统获得性抗性（SAR）信息标记的PR1基因积累的转录本。BABA预处理导致PR1基因表达极少或无表达，但先用BABA处理，随后接种致病疫霉，结果显示，应激后信息印记及其直至受精的持续时间，表现为PR1转录本积累增强以及对晚疫病的基础抗性短暂增加。易感马铃薯品种的防御引发状态在挑战接种后，作为增强的PR1 mRNA积累传递给其营养后代。然而，观察到经BABA引发处理的马铃薯基因型的不同营养株系对疾病的反应存在差异。与来自块茎的植株相比，通过离体幼苗繁殖的马铃薯虽然保留了引发的PR1基因表达，但在很大程度上丧失了诱导抗性性状。
