Lin Feng, Zhu Yuan, Hu Gangfeng
Department of Orthopedics, Xiaoshan First People's Hospital, Hangzhou, Zhejiang 310000, P.R. China.
Exp Ther Med. 2018 Oct;16(4):3504-3510. doi: 10.3892/etm.2018.6634. Epub 2018 Aug 21.
Directional migration of mesenchymal stem cells (MSCs) is known to serve roles in bone fracture healing. Naringin is a traditional medicine used in China to treat bone injury and has been confirmed to act as a chemoattractant to MSCs. In the present study, the secretion of chemokines and stimulation of relevant signaling pathways by naringin were detected to determine the molecular mechanism of naringin-induced MSC migration. In these experiments, Quantibody arrays were used to detect chemokines secreted by MSCs with or without the addition of naringin. The results revealed differential naringin-induced chemokine secretion of C-X-C motif chemokine (CXCL)5, CXCL6 and C-C motif chemokine 20. Furthermore, the Ras signaling pathway was markedly activated in the naringin-treated groups, suggesting that naringin may enhance the migrational ability of MSCs via Ras activation. Furthermore, naringin was able to promote the secretion of various chemokines derived from MSCs, which would, in turn, increase the mobility of MSCs. The aim of the present study was to provide novel candidate agents for clinical orthopedics and theoretical basis for the future improvement of adjunctive medication for bone fracture healing.
已知间充质干细胞（MSC）的定向迁移在骨折愈合中发挥作用。柚皮苷是中国用于治疗骨损伤的传统药物，并且已被证实可作为MSC的趋化因子。在本研究中，检测了柚皮苷诱导的趋化因子分泌以及相关信号通路的激活，以确定柚皮苷诱导MSC迁移的分子机制。在这些实验中，使用定量抗体阵列检测添加或不添加柚皮苷时MSC分泌的趋化因子。结果显示，柚皮苷诱导的C-X-C基序趋化因子（CXCL）5、CXCL6和C-C基序趋化因子20的趋化因子分泌存在差异。此外，在柚皮苷处理组中Ras信号通路明显激活，表明柚皮苷可能通过激活Ras增强MSC的迁移能力。此外，柚皮苷能够促进MSC衍生的各种趋化因子的分泌，这反过来又会增加MSC的迁移能力。本研究的目的是为临床骨科提供新的候选药物，并为未来改善骨折愈合辅助药物提供理论依据。
