Shao Yangjie, You Dongqi, Lou Yiting, Li Jianhua, Ying Binbin, Cheng Kui, Weng Wenjian, Wang Huiming, Yu Mengfei, Dong Lingqing
The Affiliated Stomatologic Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang Province, China.
Department of Stomatology, The Affiliated Ningbo First Hospital, Zhejiang University, Ningbo 315010, Zhejiang Province, China.
ACS Omega. 2019 Nov 6;4(21):19350-19357. doi: 10.1021/acsomega.9b02751. eCollection 2019 Nov 19.
Naringin, a Chinese herbal medicine, has been demonstrated to concentration-dependently promote osteogenic differentiation of mesenchymal stem cells (MSCs). However, it remains a challenge to load naringin on coatings for osteogenesis and further control the release kinetics. Here, we demonstrated that the release behavior of naringin on rutile nanorod films could be controlled by either mixing naringin with gelatin methacryloyl (GelMA) before spinning onto the films or soaking the obtained GelMA-incorporated films with the naringin solution to achieve the distinct degradation-type release and diffusion-type release, respectively. We further revealed that the naringin-loaded coatings facilitated adhesion, proliferation and late differentiation, and mineralization of MSCs. Our findings provided a novel strategy to engineer the coatings with controlled release of naringin and emphasized the bioactivity of naringin for the osteogenic differentiation of MSCs.
柚皮苷是一种中药材，已被证明能浓度依赖性地促进间充质干细胞（MSCs）的成骨分化。然而，将柚皮苷负载于用于成骨的涂层上并进一步控制其释放动力学仍是一项挑战。在此，我们证明，通过在旋涂到薄膜上之前将柚皮苷与甲基丙烯酰化明胶（GelMA）混合，或用柚皮苷溶液浸泡所得的含GelMA薄膜，可分别实现不同的降解型释放和扩散型释放，从而控制柚皮苷在金红石纳米棒薄膜上的释放行为。我们进一步揭示，负载柚皮苷的涂层促进了MSCs的黏附、增殖、晚期分化和矿化。我们的研究结果提供了一种设计具有可控柚皮苷释放功能涂层的新策略，并强调了柚皮苷对MSCs成骨分化的生物活性。
