Jin Zhankui, Liu Zhenguo, Xu Cuixiang, Yang Jiangcun, Xu Honghai, Chang Yanhai, Dong Xianghui, Wu Xueyuan, Sun Zhengming
Department of Orthopaedics, Shaanxi Provincial People's Hospital, Xi'an 710068, China.
Department of Intensive Care Unit, Shaanxi Provincial People's Hospital, Xi'an 710068, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2018 Jun;34(6):517-521.
Objective To investigate the relationship between caspase-9 (-Ex5+32 G/A) gene polymorphisms, Schneiderman score of magnetic resonance imaging (MRI) in lumbar disc degeneration and the expression of caspase-9 in degenerative nucleus pulposus. Methods The peripheral venous blood and prominent nucleus pulposus were obtained from 105 patients with lumbar disc herniation. Genomic DNA was extracted and analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The caspase-9(-Ex5+32 G/A) gene polymorphisms were analyzed in all DNA samples. The expression of caspase-9 in the tissues was detected by immunohistochemical SP staining. The t test was used to analyze the difference between the genotypes and the expression of caspase-9 in intervertebral disc nucleus. Pearson correlation analysis was performed to assess the association between caspase-9 expression in nucleus pulposus and the MRI score of lumbar disc. Results MRI analysis showed that the patients with AA genotype had the highest MRI scores, but there were no significant differences in MRI scores among patients with AA, GA, and GG genotypes. Compared with GG genotype carriers, AA genotype carriers had a statistically significant difference in the expression of caspase-9 in nucleus pulposus. There was no correlation between the MRI score of lumbar disc degeneration and the expression of caspase-9 in the degenerated nucleus pulposus. Conclusion The caspase-9(-Ex5+32 G/A) gene polymorphism is associated with the expression of caspase-9 in the degenerative nucleus pulposus. However, the MRI Schneiderman score is not significantly correlated with the expression of caspase-9 in degenerative nucleus pulposus.
目的 探讨半胱天冬酶-9（-Ex5+32 G/A）基因多态性、腰椎间盘退变磁共振成像（MRI）的施奈德曼评分与退变髓核中半胱天冬酶-9表达之间的关系。方法 收集105例腰椎间盘突出症患者的外周静脉血和突出的髓核。提取基因组DNA，采用基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）进行分析。对所有DNA样本分析半胱天冬酶-9（-Ex5+32 G/A）基因多态性。采用免疫组织化学SP染色法检测组织中半胱天冬酶-9的表达。采用t检验分析各基因型与椎间盘髓核中半胱天冬酶-9表达的差异。进行Pearson相关性分析，评估髓核中半胱天冬酶-9表达与腰椎间盘MRI评分之间的关联。结果 MRI分析显示，AA基因型患者的MRI评分最高，但AA、GA和GG基因型患者的MRI评分无显著差异。与GG基因型携带者相比，AA基因型携带者髓核中半胱天冬酶-9的表达有统计学显著差异。腰椎间盘退变的MRI评分与退变髓核中半胱天冬酶-9的表达无相关性。结论 半胱天冬酶-9（-Ex5+32 G/A）基因多态性与退变髓核中半胱天冬酶-9的表达相关。然而，MRI施奈德曼评分与退变髓核中半胱天冬酶-9的表达无显著相关性。
