Wang Yongfang, Xu Lianhong, Yun Zhihua, Chu Ying, Fa Zhenzhong, Deng Jianzhong, Zhang Ying, Wu Jia
Department of Clinical Laboratory, Affiliated Wujin People's Hospital, Jiangsu University, Changzhou 213002, China. *Corresponding author, E-mail:
Department of Clinical Laboratory, Affiliated Wujin People's Hospital, Jiangsu University, Changzhou 213002, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2018 Jun;34(6):546-551.
Objective To detect the frequencies of myeloid-derived suppressor cells (MDSCs) in the peripheral blood of patients with colorectal cancer (CRC) in an attempt to investigate the relationship between the change of MDSC frequencies and clinicopathologic characteristics of CRC patients. Methods The frequencies of granulocytic MDSCs (G-MDSCs) and monocytic MDSCs (M-MDSCs) in the peripheral blood of 82 CRC patients and 30 healthy volunteers were detected by flow cytometry. ANOVA and t-test were used to explore the relationship between the frequencies of MDSCs in the peripheral blood and the TNM stages, lymph node metastasis, tumor sites, histological grade as well as common therapeutic measures of CRC patients. Correlation analysis was performed to evaluate the correlation between the frequencies of G-MDSCs and M-MDSCs. Results Compared with the 30 healthy volunteers, the frequencies of G-MDSCs and M-MDSCs in the peripheral blood of the 82 CRC patients were statistically higher. The frequencies of G-MDSCs and M-MDSCs in the peripheral blood from TNF stage III and IV CRC patients were obviously higher than those from stage I and II patients, and CRC patients with lymph node metastasis had remarkably higher frequencies of G-MDSCs and M-MDSCs than the patients without lymph node metastasis. There were no statistical differences in the frequencies of G-MDSCs and M-MDSCs in the peripheral blood between CRC patients with different tumor sites as well as histological grades. Radical resection and effective adjuvant chemotherapy significantly decreased the frequencies of G-MDSCs and M-MDSCs in the peripheral blood of CRC patients. No statistical correlation was found between the frequencies of circulating G-MDSCs and M-MDSCs in CRC patients. Conclusion G-MDSC and M-MDSC frequencies in the peripheral blood of CRC patients significantly increased, and were closely associated with TNM stages and lymph node metastasis. Radical resection and effective adjuvant chemotherapy significantly reduced the frequencies of circulating G-MDSCs sand M-MDSCs of CRC patients.
目的 检测结直肠癌（CRC）患者外周血中髓源性抑制细胞（MDSCs）的频率，以探讨MDSC频率变化与CRC患者临床病理特征之间的关系。方法 采用流式细胞术检测82例CRC患者和30名健康志愿者外周血中粒细胞MDSCs（G-MDSCs）和单核细胞MDSCs（M-MDSCs）的频率。采用方差分析和t检验探讨外周血中MDSCs频率与CRC患者TNM分期、淋巴结转移、肿瘤部位、组织学分级以及常用治疗措施之间的关系。进行相关性分析以评估G-MDSCs和M-MDSCs频率之间的相关性。结果 与30名健康志愿者相比，82例CRC患者外周血中G-MDSCs和M-MDSCs的频率在统计学上更高。TNF III期和IV期CRC患者外周血中G-MDSCs和M-MDSCs的频率明显高于I期和II期患者，有淋巴结转移的CRC患者G-MDSCs和M-MDSCs的频率明显高于无淋巴结转移的患者。不同肿瘤部位和组织学分级的CRC患者外周血中G-MDSCs和M-MDSCs的频率无统计学差异。根治性切除和有效的辅助化疗显著降低了CRC患者外周血中G-MDSCs和M-MDSCs的频率。CRC患者循环G-MDSCs和M-MDSCs的频率之间未发现统计学相关性。结论 CRC患者外周血中G-MDSC和M-MDSC频率显著增加，且与TNM分期和淋巴结转移密切相关。根治性切除和有效的辅助化疗显著降低了CRC患者循环G-MDSCs和M-MDSCs的频率。
