伪狂犬病病毒多联体DNA中基因组末端及末端间连接区的序列
Sequence of the genome ends and of the junction between the ends in concatemeric DNA of pseudorabies virus.
作者信息
Harper L, Demarchi J, Ben-Porat T
出版信息
J Virol. 1986 Dec;60(3):1183-5. doi: 10.1128/JVI.60.3.1183-1185.1986.
Abstract
The nucleotide sequences of the termini of the mature pseudorabies virus genome and of the junction between these termini in concatemeric DNA were compared. To ensure conservation of unmodified 5' and 3' termini, the end fragments obtained directly (uncloned) from mature viral DNA were sequenced. The sequence obtained from 5' and 3' end labeling revealed that whereas the L terminus was blunt ended, the S terminus had a 2-base (GG) 3' overhang. The sequences spanning the junction between the termini present in concatemeric DNA was also determined and compared with that expected when the two ends of the mature DNA were juxtaposed. This comparison showed that in concatemeric DNA the ends of the mature genome had become joined by blunt-end ligation of one of the strands and that the 2-nucleotide gap on the other strand had been repaired. A significant degree of homology between the sequences spanning the junction between the ends of the varicella-zoster virus and pseudorabies virus genomes was found.
摘要
对成熟伪狂犬病病毒基因组末端的核苷酸序列以及串联体 DNA 中这些末端之间的连接处进行了比较。为确保未修饰的 5' 和 3' 末端的保守性，对直接（未克隆）从成熟病毒 DNA 获得的末端片段进行了测序。从 5' 和 3' 末端标记获得的序列显示，L 末端是平端，而 S 末端有一个 2 个碱基（GG）的 3' 突出端。还确定了串联体 DNA 中跨越末端之间连接处的序列，并与成熟 DNA 两端并列时预期的序列进行了比较。这种比较表明，在串联体 DNA 中，成熟基因组的末端通过一条链的平端连接而连接在一起，另一条链上的 2 个核苷酸缺口已被修复。发现水痘 - 带状疱疹病毒和伪狂犬病病毒基因组末端之间连接处的序列存在显著程度的同源性。
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