Lomniczi B, Blankenship M L, Ben-Porat T
J Virol. 1984 Mar;49(3):970-9. doi: 10.1128/JVI.49.3.970-979.1984.
As part of a study designed to identify the genes responsible for the virulence of pseudorabies virus, we have mapped the genomes of two independently derived vaccine strains (Bartha and Norden) by restriction enzyme analysis. The structures of these genomes have been compared with that of the genome of a laboratory strain previously mapped, of restriction fragments which had been cloned. The genome of the Bartha strain was found to be very similar to that of other pseudorabies virus strains, except that a deletion of approximately 2.7 X 10(6) daltons was found in the unique short (US) region. This deletion was also observed in the genome of the Norden vaccine strain but was not observed in the genomes of any other pseudorabies virus strains that have been studied (more than 20). The genome of the Norden strain differs from that of other pseudorabies virus strains in several other respects as well. The most important difference is that in contrast to all other pseudorabies virus strains analyzed to date, which contain a type 2 herpesvirus DNA molecule (in which the US region only inverts itself relative to the unique long [UL] region), the genome of the Norden strain is a type 3 molecule in which both the US and the UL regions of the genome invert themselves, giving rise to four isomeric forms of the genome. The ability of the UL region to invert itself is probably related to the fact that a sequence normally present in all other pseudorabies virus strains at the end of the UL only is found also in inverted form at the junction of the UL and the internal inverted repeat in the Norden strain.
作为一项旨在鉴定负责伪狂犬病病毒毒力的基因的研究的一部分，我们通过限制性酶切分析对两个独立衍生的疫苗株（Bartha和Norden）的基因组进行了图谱绘制。已将这些基因组的结构与先前绘制的实验室株的基因组结构以及已克隆的限制性片段的结构进行了比较。发现Bartha株的基因组与其他伪狂犬病病毒株的基因组非常相似，只是在独特短区域（US）发现了约2.7×10⁶道尔顿的缺失。在Norden疫苗株的基因组中也观察到了这种缺失，但在已研究的任何其他伪狂犬病病毒株（超过20种）的基因组中均未观察到。Norden株的基因组在其他几个方面也与其他伪狂犬病病毒株不同。最重要的区别是，与迄今为止分析的所有其他伪狂犬病病毒株（均含有2型疱疹病毒DNA分子，其中US区域仅相对于独特长区域[UL]自身倒转）不同，Norden株的基因组是3型分子，其中基因组的US和UL区域均自身倒转，产生基因组的四种异构体形式。UL区域自身倒转的能力可能与以下事实有关：通常仅在所有其他伪狂犬病病毒株的UL末端存在的一个序列，在Norden株的UL与内部反向重复序列的交界处也以反向形式存在。
