Crespo Cátia, Knust Elisabeth
Max-Planck-Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307, Dresden, Germany.
Max-Planck-Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307, Dresden, Germany
Biol Open. 2018 Oct 31;7(11):bio036632. doi: 10.1242/bio.036632.
Photoreceptor cells (PRCs) mature from simple epithelial cells, a process characterised by growth and compartmentalisation of the apical membrane into an inner and an outer segment. So far, a PRC subtype-specific description of morphological and cellular changes in the developing zebrafish retina is missing. Here, we performed an in-depth characterisation of four of the five PRC subtypes of the zebrafish retina between 51 and 120 h post fertilisation, including quantification of the size of different compartments, localisation of polarity proteins and positioning of organelles. One of the major findings was the anisotropic and subtype-specific growth of the different PRC compartments. In addition, a transient accumulation of endoplasmic reticulum in rod PRCs, changes in chromatin organisation in UV sensitive cones and differential expression of polarity proteins during the initial stages of PRC maturation were observed. The results obtained provide a developmental timeline that can be used as a platform for future studies on PRC maturation and function. This platform was applied to document that increased exposure to light leads to smaller apical domains of PRCs.
光感受器细胞（PRCs）由简单上皮细胞发育成熟，这一过程的特征是顶端膜生长并分隔为内段和外段。到目前为止，尚未有针对发育中的斑马鱼视网膜中PRC亚型特异性的形态学和细胞变化描述。在此，我们对受精后51至120小时之间斑马鱼视网膜的五种PRC亚型中的四种进行了深入表征，包括对不同区室大小的量化、极性蛋白的定位以及细胞器的定位。主要发现之一是不同PRC区室的各向异性和亚型特异性生长。此外，还观察到杆状PRC中内质网的短暂积累、紫外线敏感视锥细胞中染色质组织的变化以及PRC成熟初期极性蛋白的差异表达。所获得的结果提供了一个发育时间线，可作为未来PRC成熟和功能研究的平台。该平台被用于证明增加光照会导致PRC的顶端结构域变小。
