College of Biology and Food Engineering, Chongqing Three Gorges University, Wanzhou, 404100, China.
Medical School, Southeast University, Nanjing, 210009, China.
Sci Rep. 2018 Sep 20;8(1):14102. doi: 10.1038/s41598-018-32296-3.
We here employed a model animal of Caenorhabditis elegans to perform toxicity assessment of original surface water samples collected from Three Gorges Reservoir (TGR) in the quiet season in Wanzhou, Chongqing. Using some sublethal endpoints, including lifespan, body length, locomotion behavior, brood size, and intestinal reactive oxygen species (ROS) induction, we found that the examined five original surface water samples could not cause toxicity on wild-type nematodes. Nevertheless, the surface water sample collected from backwater area induced the significant increase in expressions of genes (sod-2 and sod-3) encoding Mn-SODs in wild-type nematodes. Among the examined five original surface water samples, exposure to the original surface water sample collected from backwater area could further cause the toxicity in decreasing locomotion behavior and in inducing intestinal ROS production in sod-3 mutant nematodes. Moreover, the solid phase of surface water sample collected from backwater area might mainly contribute to the observed toxicity in sod-3 mutant nematodes. Our results are helpful for understanding the potential effects of surface water in the TGR region in the quiet season on environmental organisms.
我们在这里使用秀丽隐杆线虫（Caenorhabditis elegans）作为模式动物，对重庆万州三峡水库（TGR）静水区采集的原水样品进行毒性评估。使用一些亚致死终点，包括寿命、体长、运动行为、产卵量和肠道活性氧（ROS）诱导，我们发现检测的五个原水样品对野生型线虫没有毒性。然而，来自回水区域的地表水样品诱导了编码 Mn-SOD 的基因（sod-2 和 sod-3）在野生型线虫中的表达显著增加。在检测的五个原水样品中，暴露于来自回水区域的原水样品进一步导致运动行为降低和 sod-3 突变型线虫肠道 ROS 产生的毒性。此外，来自回水区域的地表水样品的固相可能主要导致了 sod-3 突变型线虫观察到的毒性。我们的研究结果有助于理解三峡水库静水区地表水对环境生物的潜在影响。
