AtROP6 参与拟南芥缺铁胁迫响应中的活性氧信号转导。
AtROP6 is involved in reactive oxygen species signaling in response to iron-deficiency stress in Arabidopsis thaliana.
机构信息
College of Horticulture, China Agricultural University, Beijing, China.
Key Laboratory of Stress Physiology and Molecular Biology for Fruit Trees in Beijing Municipality, China Agricultural University, Beijing, China.
出版信息
FEBS Lett. 2018 Oct;592(20):3446-3459. doi: 10.1002/1873-3468.13257. Epub 2018 Oct 8.
Understanding the mechanism of iron (Fe)-deficiency responses is crucial for improving plant Fe bioavailability. Here, we found that the Arabidopsis Rho-like GTPase 6 mutant (rop6) is less sensitive to Fe-deficiency responses and has reduced levels of reactive oxygen species (ROS) compared to wild-type (WT), while AtROP6-overexpressing seedlings exhibit more sensitivity to Fe-deficiency responses and has higher levels of ROS compared to WT. Moreover, treatment with H O improves the sensitivity to Fe-deficiency responses in rop6 mutants. By using the yeast two-hybrid system, we further demonstrate the direct interaction between AtROP6 and Arabidopsis respiratory burst oxidase homolog D (AtRBOHD), which controls the generation of ROS. Overall, we suggest that AtROP6 is involved in AtRBOHD-mediated ROS signaling to modulate Fe-deficiency responses in Arabidopsis thaliana.
了解铁（Fe）缺乏响应的机制对于提高植物 Fe 生物利用度至关重要。在这里，我们发现拟南芥Rho 样 GTP 酶 6 突变体（rop6）对 Fe 缺乏响应的敏感性降低，与野生型（WT）相比，活性氧（ROS）水平降低，而 AtROP6 过表达的幼苗对 Fe 缺乏响应的敏感性增加，与 WT 相比，ROS 水平更高。此外，H2O2 处理可提高 rop6 突变体对 Fe 缺乏响应的敏感性。通过使用酵母双杂交系统，我们进一步证明了 AtROP6 与控制 ROS 产生的拟南芥呼吸爆发氧化酶同源物 D（AtRBOHD）之间的直接相互作用。总的来说，我们认为 AtROP6 参与了 AtRBOHD 介导的 ROS 信号转导，以调节拟南芥铁缺乏反应。
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