Wang Xiaoya, Zhang Zhongxing, Dong Yongjuan, Wang Yanxiu
College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China.
Plants (Basel). 2024 Aug 20;13(16):2317. doi: 10.3390/plants13162317.
The PYL protein family are crucial sensors of the core signals of abscisic acid (ABA) and significantly influence the plant's response to ABA-mediated abiotic stresses as well as its growth and development. However, research on the role of the gene in iron (Fe) deficiency in apple trees is limited. Studies have shown that the gene, when exposed to Fe-deficiency stress, exhibits more rapid transcriptional upregulation than other genes' quickly elevated transcription. However, the precise mechanism by which it alleviates this stress remains unclear. The gene (ID:103432868), isolated from , was analyzed to elucidate its function. plants engineered to overexpress the gene exhibited increased leaf chlorosis and slower growth in response to Fe stress compared to the unmodified controls. The transgenic plants also exhibited elevated levels of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities, as well as ferric chelate reductase (FCR) activities. Levels of malondialdehyde (MDA), hydrogen peroxide (HO), and superoxide anion (O) were increased. In addition, these transgenic plants had lower concentrations of proline (Pro) and Fe, which indicated that their stress tolerance was reduced. Similarly, the overexpression of in apple calli resulted in inhibited growth and increased susceptibility under Fe stress conditions. Physiological evaluations indicated that the overexpression of in reduced its Fe stress tolerance by inhibiting chlorophyll synthesis. In apple calli, it altered pH levels, antioxidant enzyme activity, and Fe-reducing capabilities under the same stress conditions. In summary, the elevated expression of the gene reduced the tolerance of both and apple calli to Fe stress, suggesting that acts as a negative regulator in response to Fe deficiency.
PYL蛋白家族是脱落酸（ABA）核心信号的关键传感器，显著影响植物对ABA介导的非生物胁迫的响应及其生长发育。然而，关于该基因在苹果树缺铁中的作用的研究有限。研究表明，该基因在缺铁胁迫下比其他基因转录上调更快。然而，其缓解这种胁迫的精确机制仍不清楚。对从[具体来源]分离的该基因（ID：103432868）进行了分析以阐明其功能。与未修饰的对照相比，经基因工程改造过表达该基因的[植物名称]在铁胁迫下表现出叶片黄化增加和生长缓慢。转基因植物还表现出超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）活性以及铁螯合还原酶（FCR）活性升高。丙二醛（MDA）、过氧化氢（HO）和超氧阴离子（O）水平增加。此外，这些转基因植物的脯氨酸（Pro）和铁浓度较低，这表明它们的胁迫耐受性降低。同样，在苹果愈伤组织中过表达该基因导致在铁胁迫条件下生长受抑制和易感性增加。生理评估表明，在[植物名称]中过表达该基因通过抑制叶绿素合成降低了其对铁胁迫的耐受性。在苹果愈伤组织中，在相同胁迫条件下它改变了pH值、抗氧化酶活性和铁还原能力。总之，该基因的高表达降低了[植物名称]和苹果愈伤组织对铁胁迫的耐受性，表明该基因在响应缺铁时起负调节作用。
