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一种归因于逆转录错误的异常Ty1结构。

An anomalous Ty1 structure attributed to an error in reverse transcription.

作者信息

Errede B, Company M, Swanstrom R

出版信息

Mol Cell Biol. 1986 Apr;6(4):1334-8. doi: 10.1128/mcb.6.4.1334-1338.1986.

DOI:10.1128/mcb.6.4.1334-1338.1986
PMID:3023883
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC367648/
Abstract

We have determined the nucleotide sequence of both delta elements of a Ty1 transposon inserted near the CYC7 gene in the Saccharomyces cerevisiae CYC7-H2 mutant. The upstream delta element in this Ty1 has an unusual inverted repeat structure that may have been formed by an error during reverse transcription.

摘要

我们已经确定了插入在酿酒酵母CYC7-H2突变体中CYC7基因附近的一个Ty1转座子的两个δ元件的核苷酸序列。这个Ty1中的上游δ元件具有不寻常的反向重复结构,可能是由逆转录过程中的一个错误形成的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66b0/367648/9190445733dc/molcellb00088-0378-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66b0/367648/9190445733dc/molcellb00088-0378-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66b0/367648/9190445733dc/molcellb00088-0378-a.jpg

相似文献

1
An anomalous Ty1 structure attributed to an error in reverse transcription.一种归因于逆转录错误的异常Ty1结构。
Mol Cell Biol. 1986 Apr;6(4):1334-8. doi: 10.1128/mcb.6.4.1334-1338.1986.
2
Identification of regulatory regions within the Ty1 transposable element that regulate iso-2-cytochrome c production in the CYC7-H2 yeast mutant.在CYC7-H2酵母突变体中鉴定Ty1转座子内调控异-2-细胞色素c产生的调控区域。
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Transcriptional analysis of Ty1 deletion and inversion derivatives at CYC7.CYC7处Ty1缺失和倒位衍生物的转录分析。
Mol Cell Biol. 1986 Oct;6(10):3299-311. doi: 10.1128/mcb.6.10.3299-3311.1986.
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Ty1 sequence with enhancer and mating-type-dependent regulatory activities.
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Ty1 transposition in Saccharomyces cerevisiae is nonrandom.酿酒酵母中的Ty1转座是非随机的。
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Activation regions in a yeast transposon have homology to mating type control sequences and to mammalian enhancers.酵母转座子中的激活区域与交配型控制序列以及哺乳动物增强子具有同源性。
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Involvement of SRE element of Ty1 transposon in TEC1-dependent transcriptional activation.Ty1转座子的SRE元件参与TEC1依赖性转录激活。
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Rearrangements occurring adjacent to a single Ty1 yeast retrotransposon in the presence and absence of full-length Ty1 transcription.在存在和不存在全长Ty1转录的情况下,单个Ty1酵母逆转录转座子附近发生的重排。
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A specific terminal structure is required for Ty1 transposition.Ty1转座需要特定的末端结构。
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RNA from the yeast transposable element Ty1 has both ends in the direct repeats, a structure similar to retrovirus RNA.来自酵母转座因子Ty1的RNA在直接重复序列中都有两端,这种结构类似于逆转录病毒RNA。
Proc Natl Acad Sci U S A. 1983 May;80(9):2432-6. doi: 10.1073/pnas.80.9.2432.

引用本文的文献

1
Ty1 sequence with enhancer and mating-type-dependent regulatory activities.
Mol Cell Biol. 1987 Jan;7(1):258-65. doi: 10.1128/mcb.7.1.258-265.1987.
2
Transcriptional analysis of Ty1 deletion and inversion derivatives at CYC7.CYC7处Ty1缺失和倒位衍生物的转录分析。
Mol Cell Biol. 1986 Oct;6(10):3299-311. doi: 10.1128/mcb.6.10.3299-3311.1986.
3
The Saccharomyces cerevisiae genome contains functional and nonfunctional copies of transposon Ty1.酿酒酵母基因组包含转座子Ty1的功能拷贝和非功能拷贝。

本文引用的文献

1
Circles with two tandem LTRs are precursors to integrated retrovirus DNA.带有两个串联长末端重复序列的环状结构是整合型逆转录病毒DNA的前体。
Cell. 1984 Mar;36(3):673-9. doi: 10.1016/0092-8674(84)90347-7.
2
Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.缓冲液梯度凝胶和35S标记辅助快速DNA序列测定。
Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5. doi: 10.1073/pnas.80.13.3963.
3
Characterization of the yeast mobile element Ty1.酵母移动元件Ty1的特性分析。
Mol Cell Biol. 1988 Apr;8(4):1432-42. doi: 10.1128/mcb.8.4.1432-1442.1988.
4
Cell-type-dependent gene activation by yeast transposon Ty1 involves multiple regulatory determinants.
Mol Cell Biol. 1987 Sep;7(9):3205-11. doi: 10.1128/mcb.7.9.3205-3211.1987.
5
The yeast ROAM mutation--identification of the sequences mediating host gene activation and cell-type control in the yeast retrotransposon, Ty.酵母ROAM突变——酵母逆转录转座子Ty中介导宿主基因激活和细胞类型控制的序列的鉴定
Nucleic Acids Res. 1987 Sep 25;15(18):7309-24. doi: 10.1093/nar/15.18.7309.
6
Characterization of trans-acting mutations affecting Ty and Ty-mediated transcription in Saccharomyces cerevisiae.影响酿酒酵母中Ty及Ty介导转录的反式作用突变的特征分析
Curr Genet. 1991 Dec;20(6):441-8. doi: 10.1007/BF00334769.
Cold Spring Harb Symp Quant Biol. 1981;45 Pt 2:609-17. doi: 10.1101/sqb.1981.045.01.079.
4
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.利用单链噬菌体克隆辅助快速DNA测序。
J Mol Biol. 1980 Oct 25;143(2):161-78. doi: 10.1016/0022-2836(80)90196-5.
5
Mating signals control expression of mutations resulting from insertion of a transposable repetitive element adjacent to diverse yeast genes.交配信号控制着因一个可转座重复元件插入到多种酵母基因附近而产生的突变的表达。
Cell. 1980 Nov;22(2 Pt 2):427-36. doi: 10.1016/0092-8674(80)90353-0.
6
The yeast transposon Ty1 generates duplications of target DNA on insertion.酵母转座子Ty1在插入时会产生靶DNA的重复序列。
Nature. 1980 Jul 24;286(5771):414-8. doi: 10.1038/286414a0.
7
Insertion of the eukaryotic transposable element Ty1 creates a 5-base pair duplication.真核转座因子Ty1的插入会产生一个5碱基对的重复序列。
Nature. 1980 Jul 24;286(5771):352-6. doi: 10.1038/286352a0.
8
Selective cleavage in the avian retroviral long terminal repeat sequence by the endonuclease associated with the alpha beta form of avian reverse transcriptase.禽逆转录酶αβ形式相关的核酸内切酶对禽逆转录病毒长末端重复序列的选择性切割。
Proc Natl Acad Sci U S A. 1983 Nov;80(22):6745-9. doi: 10.1073/pnas.80.22.6745.
9
RNA from the yeast transposable element Ty1 has both ends in the direct repeats, a structure similar to retrovirus RNA.来自酵母转座因子Ty1的RNA在直接重复序列中都有两端,这种结构类似于逆转录病毒RNA。
Proc Natl Acad Sci U S A. 1983 May;80(9):2432-6. doi: 10.1073/pnas.80.9.2432.
10
The terminal redundancy of the retrovirus genome facilitates chain elongation by reverse transcriptase.逆转录病毒基因组的末端冗余有助于逆转录酶进行链延伸。
J Biol Chem. 1981 Feb 10;256(3):1115-21.