Errede B, Cardillo T S, Sherman F, Dubois E, Deschamps J, Wiame J M
Cell. 1980 Nov;22(2 Pt 2):427-36. doi: 10.1016/0092-8674(80)90353-0.
ROAM mutations cause overproduction in S. cerevisiae. Overproduction of ROAM mutant gene products is less in MATa/MAT alpha diploid strains which cannot conjugate than in haplolid strains which can. Overproduction occurs in diploid strains capable of mating whether or not they are capable of sporulating. Overproduction decreases when haploid ROAM mutants also contain the ste7 mutation which prevents conjugation; other ste mutations do not affect the expression of ROAM mutations. Cloning of the ROAM mutant gene CYC7-H2 shows that a 5.5 kb sequence homologous to a transposable and reiterated Ty1 element is inserted in the 5' noncoding region of the CYC7 structural locus. The similar genetic properties of other ROAM mutations suggest that they each contain an inserted Ty element. These results also suggest that ROAM mutations respond to signals normally directed toward genes controlling conjugation functions, and that sequences present in Ty elements may be adjacent to structural loci and are the normal receptors for these signals.
ROAM突变导致酿酒酵母中基因产物过量产生。与可进行接合的单倍体菌株相比，不能进行接合的MATa/MATα二倍体菌株中ROAM突变基因产物的过量产生较少。在能够交配的二倍体菌株中，无论它们是否能够形成孢子，都会出现过量产生的情况。当单倍体ROAM突变体也含有阻止接合的ste7突变时，过量产生会减少；其他ste突变不影响ROAM突变的表达。对ROAM突变基因CYC7-H2的克隆表明，与可转座且重复的Ty1元件同源的5.5 kb序列插入到CYC7结构基因座的5'非编码区。其他ROAM突变的相似遗传特性表明它们各自都含有一个插入的Ty元件。这些结果还表明，ROAM突变对通常指向控制接合功能基因的信号有反应，并且Ty元件中存在的序列可能与结构基因座相邻，并且是这些信号的正常受体。
