Department of Biomedical Engineering, Duke University, Durham, North Carolina.
J Biophotonics. 2019 Feb;12(2):e201800258. doi: 10.1002/jbio.201800258. Epub 2018 Oct 12.
In recent years, significant work has been devoted to the use of angle-resolved elastic scattering for the extraction of nuclear morphology in tissue. By treating the nucleus as a Mie scattering object, techniques such as angle-resolved low-coherence interferometry (a/LCI) have demonstrated substantial success in identifying nuclear alterations associated with dysplasia. Because optical biopsies are inherently noninvasive, only a small, discretized portion of the 4π scattering field can be collected from tissue, limiting the amount of information available for diagnostic purposes. In this work, we comprehensively characterize the diagnostic impact of variations in angular sampling, range and noise for inverse light scattering analysis of nuclear morphology, using a previously reported dataset from 40 patients undergoing a/LCI optical biopsy for cervical dysplasia. The results from this analysis are applied to a benchtop scanning a/LCI system which compromises angular range for wide-area scanning capability. This work will inform the design of next-generation optical biopsy probes by directing optical design towards parameters which offer the most diagnostic utility.
近年来，人们致力于利用角度分辨弹性散射技术提取组织中的核形态。通过将细胞核视为米氏散射体，角度分辨低相干干涉（a/LCI）等技术已成功地识别出与发育不良相关的核改变。由于光学活检具有固有地非侵入性，只能从组织中收集 4π 散射场的一小部分离散部分，这限制了可用于诊断目的的信息量。在这项工作中，我们使用先前报道的 40 名接受宫颈发育不良 a/LCI 光学活检的患者数据集，全面描述了角度采样、范围和噪声变化对核形态反向光散射分析的诊断影响。该分析的结果应用于一种台式扫描 a/LCI 系统，该系统的角度范围折衷了大面积扫描能力。这项工作将通过将光学设计指向提供最具诊断效用的参数，为下一代光学活检探针的设计提供信息。
