Herbal Bioactive Research Laboratory, Faculty of Pharmacy, Integral University, Lucknow, India.
Drug Chem Toxicol. 2020 Nov;43(6):588-594. doi: 10.1080/01480545.2018.1517772. Epub 2018 Sep 21.
Alcohol is regarded as the third most common cause of death after hypertension and smoking. Its long-term excess exposure leads to alcoholic liver disease (ALD) and liver injury, a worldwide health problem without efficient therapy. As there is no reliable liver protective drugs in allopathic medical practices, herbs play a major role in the management of liver diseases. Thus, the present study was designed to evaluate hepatoprotective activity of seed extract against alcohol-induced liver injury in Sprague Dawley rats. Liver toxicity was induced by 50% alcohol at dose level of 12 ml/kg each, for 8 days. Ethanolic extract of seed (EEAS) at dose of 200 and 400 mg/kg were administered once daily for 8 consecutive days. The hepatoprotective activity of EEAS was assessed in experimental rats using various biochemical parameters like ALT, AST, ALP, LDH, SBL, albumin, total cholesterol, and total protein; and antioxidant parameters like SOD, CAT, GSH, and TBARS. It demonstrated that the treatment with EEAS significantly ( < 0.05- < 0.001) and dose-dependently prevented the alcohol-induced increase in serum levels of hepatic enzymes and significantly increased the levels of SOD, CAT, and GSH. It also significantly decreased the level of MDA. Histopathology of the liver tissues showed that EEAS attenuated the hepatocellular necrosis and led to regeneration and repair of cells toward normal. Results of this study strongly indicated the protective effect of against alcohol-induced liver injury which may be attributed to its hepatoprotective and antioxidant activities.
酒精被认为是仅次于高血压和吸烟的第三大死亡原因。长期过量饮酒会导致酒精性肝病 (ALD) 和肝损伤，这是一个全球性的健康问题，目前尚无有效的治疗方法。由于在对抗疗法中没有可靠的肝脏保护药物，因此草药在肝脏疾病的治疗中起着重要作用。因此，本研究旨在评估种子提取物对酒精诱导的 Sprague Dawley 大鼠肝损伤的保肝活性。用 50%酒精以 12ml/kg 的剂量水平每天一次，连续 8 天诱导肝毒性。种子的乙醇提取物（EEAS）以 200 和 400mg/kg 的剂量每天给药一次，连续 8 天。使用各种生化参数，如 ALT、AST、ALP、LDH、SBL、白蛋白、总胆固醇和总蛋白；和抗氧化参数，如 SOD、CAT、GSH 和 TBARS，评估 EEAS 在实验大鼠中的保肝活性。结果表明，EEAS 治疗可显著（<0.05-<0.001）和剂量依赖性地预防酒精引起的血清肝酶升高，并显著增加 SOD、CAT 和 GSH 的水平。它还显著降低了 MDA 的水平。肝组织病理学显示，EEAS 减轻了肝细胞坏死，并促使细胞向正常方向再生和修复。这项研究的结果强烈表明了对酒精性肝损伤的保护作用，这可能归因于其保肝和抗氧化活性。
