Nishiuchi Mika, Sakai Kumiko, Tajima Hiroyuki, Katayama Kazuo, Kimura Fumiko, Hoshi Seiko, Goto Tomoko, Shirakawa Hitoshi, Komai Michio
a Laboratory of Nutrition, Graduate School of Agricultural Science , Tohoku University , Sendai , Japan.
b Department of Human Health and Nutrition , Shokei Gakuin University , Miyagi , Japan.
Biosci Biotechnol Biochem. 2018 Dec;82(12):2168-2175. doi: 10.1080/09168451.2018.1516543. Epub 2018 Sep 21.
We previously reported an orexigenic action of oral zinc administration in male Sprague-Dawley (SD) rats during an early stage of feeding with a zinc-deficient diet, without decreased zinc concentrations in tissues. The overall conclusion was that orally but not intraperitoneally administered zinc stimulates food intake in short-term zinc-deficient-diet fed rats. We here investigate the mechanism of the orexigenic action of zinc using GC-MS/MS-targeted metabolomic analysis in the rat hypothalamus. Four-week-old, male SD/Slc rats were used, and after 2 days of feeding with a zinc-deficient diet, 3 mg of ZnSO in 5 mL saline solution were administered to each rat either orally or intraperitoneally. Three hours after administration, the rats were sacrificed and the hypothalamus were excised and analyzed. We found that the oral administration group showed increased concentrations of 3-aminopropanoic acid (β-alanine), hypotaurine, dopamine, and biotin. In light of metabolomic analysis of these results, we indicate directions for further research.
我们之前报道过，在给雄性斯普拉格-道利（SD）大鼠喂食缺锌饮食的早期阶段，口服锌具有促食欲作用，且组织中的锌浓度并未降低。总体结论是，口服而非腹腔注射锌会刺激短期喂食缺锌饮食大鼠的食物摄入量。我们在此利用气相色谱-串联质谱靶向代谢组学分析研究大鼠下丘脑锌促食欲作用的机制。使用4周龄的雄性SD/Slc大鼠，在喂食缺锌饮食2天后，每只大鼠经口或腹腔注射5 mL盐溶液中的3 mg硫酸锌。给药3小时后，处死大鼠并切除下丘脑进行分析。我们发现口服给药组中3-氨基丙酸（β-丙氨酸）、亚牛磺酸、多巴胺和生物素的浓度增加。根据对这些结果的代谢组学分析，我们指出了进一步研究的方向。
