Department of Biomedical Engineering, The University of Michigan, Ann Arbor, MI, United States of America.
Department of Biologic and Materials Sciences, The University of Michigan, Ann Arbor, MI, United States of America.
PLoS One. 2018 Sep 21;13(9):e0204470. doi: 10.1371/journal.pone.0204470. eCollection 2018.
Exercise has long-lasting benefits to bone mass and structural strength even after cessation. Combining exercise with a calcium- and phosphorus-supplemented diet increases cortical bone mineral content (BMC), area, and yield force more than exercise alone in adult mice. These increases could also be maintained after stopping exercise if the modified diet is maintained. It was hypothesized that combining exercise with a mineral-supplemented diet would lead to greater cortical BMC, area, and yield force immediately after a lengthy exercise program and after an equally long period of non-exercise (detraining) in adult mice. Male, 16-week old C57Bl/6 mice were assigned to 9 weight-matched groups-a baseline group, exercise and non-exercise groups fed a control or mineral-supplemented diet for 8 weeks, exercise + detraining and non-exercise groups fed a control or mineral-supplemented diet for 16 weeks. Exercise + detraining consisted of 8 weeks of exercise followed by 8 weeks without exercise. The daily exercise program consisted of running on a treadmill at 12 m/min, 30 min/day. After 8 weeks, mice fed the supplemented diet had greater tibial cortical BMC and area, trabecular bone volume/tissue volume (BV/TV), bone mineral density (vBMD), yield force, and ultimate force than mice fed the control diet. Exercise increased cortical BMC and area only when coupled with the supplemented diet. After 16 weeks, both exercised and non-exercised mice fed the supplemented diet maintained greater tibial cortical BMC and area, trabecular BV/TV, vBMD, yield force, and ultimate force than mice fed the control diet. Combining exercise with a mineral-supplemented diet leads to greater bone mass and structural strength than exercise alone. These benefits remain after an equally long period of detraining. Long-term use of dietary mineral supplements may help increase and maintain bone mass with aging in adult mice.
运动对骨量和结构强度有持久的益处，即使停止运动后也是如此。在成年小鼠中，将运动与钙和磷补充饮食相结合比单独运动更能增加皮质骨矿物质含量（BMC）、面积和屈服力。如果保持改良饮食，这些增加在停止运动后也可以维持。研究假设，将运动与矿物质补充饮食相结合，会导致成年小鼠在进行长时间的运动计划后和同样长时间的非运动（停训）后，皮质骨 BMC、面积和屈服力更大。雄性、16 周龄 C57Bl/6 小鼠被分为 9 个体重匹配组-基线组、运动和非运动组，分别喂食对照或矿物质补充饮食 8 周，运动+停训和非运动组喂食对照或矿物质补充饮食 16 周。运动+停训包括 8 周的运动，然后是 8 周没有运动。每日运动方案包括在跑步机上以 12m/min 的速度跑步，每天 30 分钟。8 周后，喂食补充饮食的小鼠胫骨皮质 BMC 和面积、小梁骨体积/组织体积（BV/TV）、骨矿物质密度（vBMD）、屈服力和最大力均大于喂食对照饮食的小鼠。只有当与补充饮食相结合时，运动才会增加皮质 BMC 和面积。16 周后，喂食补充饮食的运动和非运动小鼠均保持胫骨皮质 BMC 和面积、小梁 BV/TV、vBMD、屈服力和最大力大于喂食对照饮食的小鼠。将运动与矿物质补充饮食相结合比单独运动能带来更大的骨量和结构强度。这些益处在同样长的停训期后仍然存在。长期使用膳食矿物质补充剂可能有助于增加和维持成年小鼠的骨量。
