Rohde G C, Venezian E, Huidobro-Toro J P
Neurosci Lett. 1986 Nov 11;71(2):197-202. doi: 10.1016/0304-3940(86)90558-6.
The distribution of purinergic and adrenergic responses in the epididymal and prostatic segment of the rat vas deferens were studied in vitro. Prazosin antagonizes the twitch elicited by electrical stimulation mainly in the epididymal segment while alpha,beta-methyleneadenosine 5'-triphosphate (alpha,beta-mATP) preferentially inhibits the response of the prostatic segment. Using both prazosin plus alpha,beta-mATP, the response to field stimulation was completely inhibited. Concentration-response curves revealed that adrenergic compounds elicited a greater contraction in the epididymal portion than in the prostatic end of the ductus. Purinergic compounds caused a contraction of greater magnitude in the prostatic portion. The results suggest that adrenergic and purinergic mechanisms are asymmetrically distributed along the vas deferens reflecting a gradient of adrenergic and purinergic receptors along the ductus.
在体外研究了大鼠输精管附睾段和前列腺段中嘌呤能和肾上腺素能反应的分布。哌唑嗪主要在附睾段拮抗电刺激引起的抽搐，而α,β-亚甲基腺苷5'-三磷酸（α,β-mATP）优先抑制前列腺段的反应。使用哌唑嗪加α,β-mATP，可完全抑制对场刺激的反应。浓度-反应曲线显示，肾上腺素能化合物在附睾部分引起的收缩比在输精管前列腺端更大。嘌呤能化合物在前列腺部分引起的收缩幅度更大。结果表明，肾上腺素能和嘌呤能机制沿输精管不对称分布，反映了沿输精管的肾上腺素能和嘌呤能受体梯度。
