Laboratory of Fish Biogenetics & Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, China.
Laboratory of Fish Biogenetics & Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, China.
Fish Shellfish Immunol. 2018 Dec;83:406-409. doi: 10.1016/j.fsi.2018.09.050. Epub 2018 Sep 18.
Apoptosis is a basic biological phenomenon of cells, which is an important component in the evolution of organisms, the stabilization of the internal environment and the development of multiple systems. In addition, the caspase protein family plays an important role in these pathways of apoptosis. Among them, apoptotic executors can directly act on specific substrates to complete the apoptotic response. In this study, we identified the Caspase-6 and Caspase-7 genes of miiuy croaker, and then analyzed the evolution of the whole Caspase family, furthermore described the evolutionary selection sites of the caspase-6 and caspase-7 genes in fish. The results showed that Caspase-6 gene appeared earlier than Caspase-7 in species evolution and gene duplication in teleost fish. Moreover, we also found that caspase-6 gene had no potential positive selection sites in the evolution of fish. Unlike the caspase-6 gene, the caspase-7 gene did not appear to be missed or replicated during the evolution of the species, while, it to be found two potential positive selection sites.
细胞凋亡是细胞的一种基本生物学现象，是生物进化、内环境稳定以及多系统发育的重要组成部分。此外，半胱氨酸天冬氨酸蛋白酶蛋白家族在这些凋亡途径中发挥着重要作用。其中，凋亡执行者可以直接作用于特定的底物，完成凋亡反应。本研究鉴定了小黄鱼的 Caspase-6 和 Caspase-7 基因，然后分析了整个 Caspase 家族的进化，进一步描述了 caspase-6 和 caspase-7 基因在鱼类中的进化选择位点。结果表明，在种系进化和硬骨鱼基因复制中，Caspase-6 基因比 Caspase-7 基因出现得更早。此外，我们还发现 caspase-6 基因在鱼类进化过程中没有潜在的正选择位点。与 caspase-6 基因不同，caspase-7 基因在物种进化过程中似乎没有缺失或复制，而在进化过程中发现了两个潜在的正选择位点。
