Cao Jie, Fang Dan, Qiu Weiqiang, Xie Jing
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
National Experimental Teaching Demonstration Center for Food Science and Engineering, Shanghai Ocean University, Shanghai 201306, China.
Animals (Basel). 2024 Dec 12;14(24):3583. doi: 10.3390/ani14243583.
Live fish transportation plays a crucial role in the commercial fish trade. Consequently, mitigating stress during transportation is essential for enhancing the survival rate of fish and reducing potential financial losses. In this study, the effectiveness was evaluated of exogenous tryptophan in reducing transport stress in hybrid grouper, ♀ × ♂. Firstly, the groupers were divided into the following five experimental groups: 40 mg/L MS-222 group, 30 mg/L tryptophan, 50 mg/L tryptophan, 70 mg/L tryptophan, and the control group without additives. Followed by transportation simulation, the fish samples were collected before and after transportation for the determination of antioxidant enzyme activities, apoptosis gene, and inflammatory gene expressions. The results indicated that the superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities and malondialdehyde (MDA) levels in all groups were significantly increased, while they were lower in the 50 mg/L Trp treated group compared to the control group ( < 0.05). Compared with the control group in the 50 mg/L Trp, 70 mg/L Trp, and 40 mg/L MS-222-treated groups, serum cortisol and blood glucose levels were significantly increased ( < 0.05), and anti-inflammatory factor () gene expression was upregulated and pro-inflammatory factor () gene expression was decreased ( < 0.05). In addition, it was found that the 30 mg/L Trp, 50 mg/L Trp, and 40 mg/L MS-222 treatment groups had less green fluorescence than the control group by measuring the mitochondrial membrane potential, and 50 mg/L Trp and MS-222 showed more red fluorescence in fluorescence images than the other samples at the same sampling time. Therefore, in this study, it was demonstrated that the tryptophan could be used as a new anti-stress agent for hybrid groupers during transport, and additional research is required to identify the specific conditions that yield the best outcomes.
活鱼运输在商业鱼类贸易中起着至关重要的作用。因此，减轻运输过程中的应激对于提高鱼类存活率和减少潜在经济损失至关重要。在本研究中，评估了外源性色氨酸对降低杂交石斑鱼（♀×♂）运输应激的效果。首先，将石斑鱼分为以下五个实验组：40mg/L MS - 222组、30mg/L色氨酸组、50mg/L色氨酸组、70mg/L色氨酸组以及无添加剂的对照组。随后进行运输模拟，在运输前后采集鱼样本，用于测定抗氧化酶活性、凋亡基因和炎症基因表达。结果表明，所有组的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH - Px）活性以及丙二醛（MDA）水平均显著升高，而50mg/L色氨酸处理组与对照组相比更低（<0.05）。与对照组相比，50mg/L色氨酸、70mg/L色氨酸和40mg/L MS - 222处理组的血清皮质醇和血糖水平显著升高（<0.05），抗炎因子（）基因表达上调，促炎因子（）基因表达下降（<0.05）。此外，通过测量线粒体膜电位发现，30mg/L色氨酸、50mg/L色氨酸和40mg/L MS - 222处理组的绿色荧光比对照组少，并且在相同采样时间的荧光图像中，50mg/L色氨酸和MS - 222显示的红色荧光比其他样本更多。因此，在本研究中证明，色氨酸可作为杂交石斑鱼运输过程中的新型抗应激剂，但需要进一步研究以确定产生最佳效果的具体条件。
