Maeda Yuuki, Miwa Yoko, Sato Iwao
Division of Anatomy, Nippon Dental University Graduate School of Life Dentistry, Tokyo, Japan; Department of Anatomy, School of Life Dentistry at Tokyo, The Nippon Dental University, Tokyo, Japan.
Department of Anatomy, School of Life Dentistry at Tokyo, The Nippon Dental University, Tokyo, Japan.
Ann Anat. 2019 Jan;221:38-47. doi: 10.1016/j.aanat.2018.09.001. Epub 2018 Sep 18.
Calcitonin gene-related peptide-α (CGRPα) is a neurotransmitter that is related to bone formation during development. However, CGRP expression is not well known to affect the formation of teeth during development. During tooth germ development, the relationships among CGRPα, calcitonin receptor-like receptor (CRLR), amelogenin (AMELX), dentin sialophosphoprotein (DSPP), osteopontin (OPN) and osteocalcin (OCN) are unclear despite various tooth and osteogenesis markers. Our real-time RT-PCR results showed that the expression levels of CGRPα mRNA gradually decreased, in contrast to the mRNA abundances of CRLR, AMELX, DSPP, OPN, and OCN, which rapidly increased from E14.5 to P1 in the mandible. In situ hybridization using an antisense probe for CGRPα mRNA showed significant localized expression levels around the tooth bud at E14.5 and epithelial cells near the dental ledge and outer and inner enamel epithelium at E17.5 compared to those at P1. The localization of the anti-CGRPα antibody reaction revealed a strong positive reaction at the surface layer of oral epithelial cells at E14.5 and oral epithelial cells of the dental lamina around the dental ledge depression in the mandible of E17.5 mice using immunohistochemical methods The different anti-CGRPα reaction revealed its important roles during tooth formation at the postnatal stage. CGRPα mRNA was also detected in the interactions of tooth germ with the formation of odontoblast and amelobast layers from dental papilla and inner enamel epithelium. CGRPα may also be related to tooth germ development. Furthermore, CGRPα is an important tooth and bone formation marker, and bone cells provide further evidence of a role in mandibular development in contrast to inflammatory systems.
降钙素基因相关肽-α（CGRPα）是一种在发育过程中与骨形成相关的神经递质。然而，CGRP的表达对牙齿发育过程中牙齿形成的影响尚不明确。在牙胚发育过程中，尽管有各种牙齿和成骨标记物，但CGRPα、降钙素受体样受体（CRLR）、釉原蛋白（AMELX）、牙本质涎磷蛋白（DSPP）、骨桥蛋白（OPN）和骨钙素（OCN）之间的关系仍不清楚。我们的实时逆转录聚合酶链反应结果显示，与CRLR、AMELX、DSPP、OPN和OCN的mRNA丰度相反，CGRPα mRNA的表达水平逐渐下降，这些基因的mRNA丰度在下颌骨中从胚胎第14.5天到出生后第1天迅速增加。使用针对CGRPα mRNA的反义探针进行原位杂交显示，与出生后第1天相比，在胚胎第14.5天牙胚周围以及胚胎第17.5天牙板附近的上皮细胞、外釉上皮和内釉上皮中，CGRPα mRNA有显著的局部表达水平。使用免疫组织化学方法，抗CGRPα抗体反应的定位显示，在胚胎第14.5天口腔上皮细胞表层以及胚胎第17.5天小鼠下颌骨牙板凹陷周围的牙板口腔上皮细胞中有强烈的阳性反应。不同的抗CGRPα反应揭示了其在出生后牙齿形成过程中的重要作用。在牙胚与牙乳头和内釉上皮形成成牙本质细胞层和成釉细胞层的相互作用中也检测到了CGRPα mRNA。CGRPα也可能与牙胚发育有关。此外，CGRPα是一种重要的牙齿和骨形成标记物，与炎症系统相比，骨细胞为其在下颌骨发育中的作用提供了进一步的证据。
