感觉神经通过Wnt5a信号传导调节干细胞稳态。
The sensory nerve regulates stem cell homeostasis through Wnt5a signaling.
作者信息
Zhang Ting, Liu Jiaying, Jin Weiqiu, Nie Hua, Chen Sheng, Tang Xuna, Liu Rong, Wang Min, Chen Rixin, Lu Jiangyue, Bao Jun, Jiang Shaoyun, Xiao Yin, Yan Fuhua
机构信息
Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology, Nanjing University, Nanjing, China.
School of Medicine and Dentistry, Griffith University, Gold Coast, QLD 4222, Australia.
出版信息
iScience. 2024 Sep 26;27(10):111035. doi: 10.1016/j.isci.2024.111035. eCollection 2024 Oct 18.
Increasing evidence indicates that nerves play a significant role in regulating stem cell homeostasis and developmental processes. To explore the impact of nerves on epithelial stem cell homeostasis during tooth development, the regulation of sensory nerves on stem cell homeostasis was investigated using a rat model of incisor development. Impaired mineralization, decreased enamel thickness, and fractured enamel rods of the incisor were observed after denervation. qPCR and histological staining revealed that the expression of enamel-related factors ameloblastin (AMBN), kallikrein-4, amelogenin (Amelx), collagen type XVII (col17a), and enamelin were decreased in the incisor enamel of rats with sensory nerve injure. The decreased expression of Wnt5a in ameloblasts was coupled with the downregulation of calcium ion-related calmodulin kinase II. These results implicate that the sensory nerves are essential in stem cell homeostasis for enamel mineralization and development.
越来越多的证据表明，神经在调节干细胞稳态和发育过程中发挥着重要作用。为了探究牙齿发育过程中神经对上皮干细胞稳态的影响，利用大鼠切牙发育模型研究了感觉神经对干细胞稳态的调节作用。去神经支配后观察到切牙矿化受损、牙釉质厚度降低和牙釉质棒断裂。定量聚合酶链反应（qPCR）和组织学染色显示，感觉神经损伤大鼠的切牙牙釉质中，牙釉质相关因子成釉蛋白（AMBN）、激肽释放酶-4、釉原蛋白（Amelx）、ⅩⅦ型胶原（col17a）和釉蛋白的表达降低。成釉细胞中Wnt5a表达的降低与钙离子相关的钙调蛋白激酶II的下调有关。这些结果表明，感觉神经对于牙釉质矿化和发育的干细胞稳态至关重要。
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